Association of antinuclear and antinucleolar antibodies in progressive systemic sclerosis

Antinuclear and/or antinucleolar antibodies were demonstrated in the sera of 74 of 76 patients (97%) with progressive systemic sclerosis, using tissue culture cells (HEp-2) as substrate in the indirect immunofluorescent method. Six patterns of nuclear staining and three nucleolar patterns were recog...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and experimental immunology 1982-04, Vol.48 (1), p.43-51
Main Authors: Bernstein, R M, Steigerwald, J C, Tan, E M
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies, Antinuclear - analysis
Calcinosis - complications
Cell Nucleolus - enzymology
Cell Nucleolus - immunology
Cell Nucleus - enzymology
Cell Nucleus - immunology
Centromere
Esophageal Diseases - complications
Female
Fluorescent Antibody Technique
Humans
Middle Aged
Precipitins - analysis
Rabbits
Raynaud Disease - complications
Rheumatoid Factor - analysis
Scleroderma, Systemic - complications
Scleroderma, Systemic - enzymology
Scleroderma, Systemic - immunology
Syndrome
Telangiectasis - complications
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Antinuclear and/or antinucleolar antibodies were demonstrated in the sera of 74 of 76 patients (97%) with progressive systemic sclerosis, using tissue culture cells (HEp-2) as substrate in the indirect immunofluorescent method. Six patterns of nuclear staining and three nucleolar patterns were recognized. The nuclear patterns were centromere, fine speckles, coarse speckles, diffusely grainy, homogeneous and nuclear dots. The nucleolar patterns were speckled, homogeneous and clumpy. The results of digestion studies with ribonuclease, deoxyribonuclease and trypsin suggested that the nuclear antigens are proteins, some of which may be associated with chromatin. The nucleolar antigens appeared to be nucleic acid in nature. Certain characteristic serologic and clinical features associated with staining patterns were observed. The diffusely grainy pattern was seen only in sera containing antibody to Scl-70 antigen. Centromere staining was confirmed to be highly selective for the CREST (Calcinosis, Raynaud's phenomenon, esophageal involvement, sclerodactyly and telangiectasis) variant of progressive systemic sclerosis with rheumatoid factor titres higher in these patients with anti-centromere antibodies.
ISSN:0009-9104
1365-2249