Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens

Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal condi...

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Bibliographic Details
Published in:Clinical and experimental immunology 1980-08, Vol.41 (2), p.309-316
Main Authors: Zembala, M, Lemmel, E M, Uracz, W
Format: Article
Language:English
Subjects:
Cell Adhesion
Cells, Cultured
Esterases - metabolism
Humans
Lymphocyte Activation
Monocytes - enzymology
Monocytes - immunology
Nitroblue Tetrazolium
Phagocytosis
Phytohemagglutinins - pharmacology
Receptors, Fc - analysis
Zymosan
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Summary:Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal conditions is 12.2 +/- 2.4 x 10(-9) mol . hr-1 . 10(-6) monocytes. Monocytes which have phagocytosed zymosan depress the mitogen response of human lymphocytes to PHA. This effect of 'activated' monocytes is due to a soluble inhibitory mediator which appears in the supernatant after culture for 24 hr. Its appearance requires protein synthesis. It is suggested that NBT reduction of peripheral blood mononuclear cells can be used as a test for the state of monocyte activation in disease. The possibility that activated monocytes may depress blast transformation in vitro in disease states is discussed.
ISSN:0009-9104
1365-2249