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Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens

Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal condi...

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Published in:	Clinical and experimental immunology 1980-08, Vol.41 (2), p.309-316
Main Authors:	Zembala, M, Lemmel, E M, Uracz, W
Format:	Article
Language:	English
Subjects:	Cell Adhesion Cells, Cultured Esterases - metabolism Humans Lymphocyte Activation Monocytes - enzymology Monocytes - immunology Nitroblue Tetrazolium Phagocytosis Phytohemagglutinins - pharmacology Receptors, Fc - analysis Zymosan
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creator	Zembala, M Lemmel, E M Uracz, W
description	Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal conditions is 12.2 +/- 2.4 x 10(-9) mol . hr-1 . 10(-6) monocytes. Monocytes which have phagocytosed zymosan depress the mitogen response of human lymphocytes to PHA. This effect of 'activated' monocytes is due to a soluble inhibitory mediator which appears in the supernatant after culture for 24 hr. Its appearance requires protein synthesis. It is suggested that NBT reduction of peripheral blood mononuclear cells can be used as a test for the state of monocyte activation in disease. The possibility that activated monocytes may depress blast transformation in vitro in disease states is discussed.
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This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal conditions is 12.2 +/- 2.4 x 10(-9) mol . hr-1 . 10(-6) monocytes. Monocytes which have phagocytosed zymosan depress the mitogen response of human lymphocytes to PHA. This effect of 'activated' monocytes is due to a soluble inhibitory mediator which appears in the supernatant after culture for 24 hr. Its appearance requires protein synthesis. It is suggested that NBT reduction of peripheral blood mononuclear cells can be used as a test for the state of monocyte activation in disease. The possibility that activated monocytes may depress blast transformation in vitro in disease states is discussed.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>PMID: 7438557</identifier><language>eng</language><publisher>England</publisher><subject>Cell Adhesion ; Cells, Cultured ; Esterases - metabolism ; Humans ; Lymphocyte Activation ; Monocytes - enzymology ; Monocytes - immunology ; Nitroblue Tetrazolium ; Phagocytosis ; Phytohemagglutinins - pharmacology ; Receptors, Fc - analysis ; Zymosan</subject><ispartof>Clinical and experimental immunology, 1980-08, Vol.41 (2), p.309-316</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1537002/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1537002/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7438557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zembala, M</creatorcontrib><creatorcontrib>Lemmel, E M</creatorcontrib><creatorcontrib>Uracz, W</creatorcontrib><title>Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). This reduction is due to monocytes, i.e. adherent, phagocytic, esterase-positive cells with Fc receptors. Monocytes allowed to phagocytose zymosan show increased NBT reduction which under optimal conditions is 12.2 +/- 2.4 x 10(-9) mol . hr-1 . 10(-6) monocytes. Monocytes which have phagocytosed zymosan depress the mitogen response of human lymphocytes to PHA. This effect of 'activated' monocytes is due to a soluble inhibitory mediator which appears in the supernatant after culture for 24 hr. Its appearance requires protein synthesis. It is suggested that NBT reduction of peripheral blood mononuclear cells can be used as a test for the state of monocyte activation in disease. The possibility that activated monocytes may depress blast transformation in vitro in disease states is discussed.</description><subject>Cell Adhesion</subject><subject>Cells, Cultured</subject><subject>Esterases - metabolism</subject><subject>Humans</subject><subject>Lymphocyte Activation</subject><subject>Monocytes - enzymology</subject><subject>Monocytes - immunology</subject><subject>Nitroblue Tetrazolium</subject><subject>Phagocytosis</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Receptors, Fc - analysis</subject><subject>Zymosan</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><recordid>eNpVkE1LxDAQhoMo67r6E4ScvBXSJk22F2FZ_ALBi55Lmk63kSapTbK4_nrDWkRPw3w97ztzgpY55WVWFKw6RUtCSJVVOWHn6ML795RyzosFWghG12Upluhzo4Ley6Cdxa7DfTTSYuOsU4cAHnduwlaHyTVDBBwgTPLLDToaPEEb1XFN2haHHrCP4ziB9zNqOJixP2LSrB-d9QngsNHB7cD6S3TWycHD1RxX6O3-7nX7mD2_PDxtN8_ZWPA8ZKztJIiWSJq8q5IpWghZNBIUT_5bxruOrRuupOREVEzkgqgiVTpFAcoK6Ard_nDH2BhoFdh0w1CPkzZyOtRO6vp_x-q-3rl9nZdUEFIkwM0MmNxHBB9qo72CYZAWXPS1KGlJWU7T4PVfpV-J-df0G5K2gXk</recordid><startdate>19800801</startdate><enddate>19800801</enddate><creator>Zembala, M</creator><creator>Lemmel, E M</creator><creator>Uracz, W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800801</creationdate><title>Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens</title><author>Zembala, M ; Lemmel, E M ; Uracz, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-4dfae7d0a3006c54c327a2baec6557d46ff48b6caa607947170c248bfc3ee59e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Cell Adhesion</topic><topic>Cells, Cultured</topic><topic>Esterases - metabolism</topic><topic>Humans</topic><topic>Lymphocyte Activation</topic><topic>Monocytes - enzymology</topic><topic>Monocytes - immunology</topic><topic>Nitroblue Tetrazolium</topic><topic>Phagocytosis</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Receptors, Fc - analysis</topic><topic>Zymosan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zembala, M</creatorcontrib><creatorcontrib>Lemmel, E M</creatorcontrib><creatorcontrib>Uracz, W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zembala, M</au><au>Lemmel, E M</au><au>Uracz, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1980-08-01</date><risdate>1980</risdate><volume>41</volume><issue>2</issue><spage>309</spage><epage>316</epage><pages>309-316</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><abstract>Human peripheral blood mononuclear cells freed from polymorphs reduce nitroblue tetrazolium (NBT). 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subjects	Cell Adhesion Cells, Cultured Esterases - metabolism Humans Lymphocyte Activation Monocytes - enzymology Monocytes - immunology Nitroblue Tetrazolium Phagocytosis Phytohemagglutinins - pharmacology Receptors, Fc - analysis Zymosan
title	Activation of human monocytes for nitroblue tetrazolium reduction and the suppression of lymphocyte response to mitogens
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