Demonstration of increased anti-mycobacterial activity in peripheral blood monocytes after BCG vaccination in British school children

A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks after. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low...

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Bibliographic Details
Published in:Clinical and experimental immunology 1988-10, Vol.74 (1), p.20-25
Main Authors: SHUK HAN CHENG, WALKER, L, POOLE, J, ABER, V. R, WALKER, K. B, MITCHISON, D. A, LOWRIE, D. B
Format: Article
Language:English
Subjects:
Adolescent
Applied microbiology
BCG Vaccine - immunology
Biological and medical sciences
Colony Count, Microbial
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Leukocyte Count
Lymphocyte Activation
Macrophages - immunology
Microbiology
Mycobacterium - immunology
Mycobacterium - isolation & purification
Mycobacterium microti
Time Factors
Tuberculosis - prevention & control
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks after. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low bacterium/monocyte ratio and allowed to form monolayers in microtitre wells. The infected monolayers were rinsed daily and the change in number of live bacteria in monolayers and supernatants was monitored by colony counts on agar. The cells were bacteriostatic during the first day, thereafter growth accelerated in pre-vaccination monolayers. When monolayers received pulsed exposures to autologous lymphocytes that had been incubated with whole dead tubercle bacilli the growth rates of M. microti were increased. However, growth rates in lymphocyte-pulsed monolayers were significantly lower after vaccination than before. It is proposed that this difference reflects the protective effect of vaccination.
ISSN:0009-9104
1365-2249