Fibronectin (FN) decreases glomerular lesions and synthesis of tumour necrosis factor‐alpha (TNF‐α), platelet‐activating factor (PAF) and FN in proliferative glomerulonephritis

• Published in: Clinical and experimental immunology 1995-08, Vol.101 (2), p.334-340
• Main Authors: ORTÍZ, A., ALONSO, J., GÓMEZ‐CHIARRI, M., LERMA, J. L., SERON, D., CONDOM, E., GONZÁLEZ, E., EGIDO, J.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; fibronectin; Fibronectins - biosynthesis; Fibronectins - pharmacology; Glomerular Mesangium - drug effects; Glomerular Mesangium - metabolism; Glomerulonephritis; Glomerulonephritis, Membranoproliferative - drug therapy; Glomerulonephritis, Membranoproliferative - metabolism; Kidney - cytology; Kidney - metabolism; Leukocytes, Mononuclear - metabolism; Male; Medical sciences; necrosis factor; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Neutrophils - metabolism; Platelet Activating Factor - biosynthesis; platelet‐activating factor; proteinuria; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha - biosynthesis; tumour
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.1995.tb08360.x

SUMMARY We have studied the effect of therapy with plasma FN on glomerular synthesis of PAF, TNF‐α and FN, in experimental proliferative glomerulonephritis. Glomerular PAF, TNF‐α and FN production were increased in rats with nephritis. Peak glomerular PAF production preceded, while peak glomerular TNF‐α bioactivity coincided with maximal proteinuria. Rats treated with FN (5 mg/kg per 48 h) for 15 days had less proteinuria, glomerular and interstitial cell infiltration and glomerular PAF, TNF‐α and FN synthesis than non‐treated rats. In order to characterize further the mechanisms of action of FN, healthy rats were injected with either FN or saline. Peripheral blood mononuclear cells and neutrophils from healthy rats injected with FN secreted less TNF‐α and PAF, respectively, than those obtained from saline‐treated rats. Our data suggest that the beneficial effect of FN may be related to decreased number of glomerular leucocytes and decreased synthesis of inflammatory mediators and extracellular matrix.