Progressive renal lesions induced by administration of monoclonal antibody 1‐22‐3 to unilaterally nephrectomized rats

SUMMARY A new animal model of progressive glomerulosclerosis was developed by administering a single i.v., injection of MoAb 1–22–3 to unilaterally nephrectomized rats. Renal morphological analysis revealed that glomerular lesions characterized by mesangial cell proliferation and mesangial matrix ex...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and experimental immunology 1995-10, Vol.102 (1), p.181-185
Main Authors: CHENG, Q.L., ORIKASA, M., MORIOKA, T., KAWACHI, H., CHEN, X.M., OITE, T., SHIMIZU, F.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
antibody
Biological and medical sciences
Chronic Disease
Female
Glomerulonephritis
Glomerulonephritis - etiology
Glomerulonephritis - pathology
glomerulosclerosis
Kidney - pathology
Medical sciences
monoclonal
Nephrectomy
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Platelet-Derived Growth Factor - analysis
proteinuria
Proteinuria - etiology
Rats
Rats, Wistar
Transforming Growth Factor beta - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SUMMARY A new animal model of progressive glomerulosclerosis was developed by administering a single i.v., injection of MoAb 1–22–3 to unilaterally nephrectomized rats. Renal morphological analysis revealed that glomerular lesions characterized by mesangial cell proliferation and mesangial matrix expansion were induced in about 95% of the glomeruli. Approximately 20% of the glomeruli of the unilaterally nephrectomized rats showed sclerosis or segmental sclerosis by week 6 after MoAb injection and crescent formation was observed in some glomeruli (ca 4%). Cellular infiltration was also noted in some parts of the interstitium. Increased expression of transforming growth factor–beta (TGF‐β) was observed in the unilaterally nephrectomized rats treated with MoAb 1‐22‐3, but we could not demonstrate pathological involvement of platelet‐derived growth factor (PDGF). even though early‐stage mesangial cell proliferation was observed. The mechanism of mesangial cell proliferation in this model remains to be elucidated. The relatively short period of time needed to induce the sclerotic changes is considered to be a great advantage of this model for clarifying the mechanisms involved in the chronic progression of mesangial proliferative glomerulonephritis.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1995.tb06653.x