Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression

Specific recruitment of corepressor complexes containing histone deacetylases (HDAC) by transcription factors is believed to play an essential role in transcriptional repression. Recent studies indicate that repression by unliganded nuclear hormone receptors and by the Mad family of repressors requi...

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Bibliographic Details
Published in:Genes & development 2002-03, Vol.16 (6), p.687-692
Main Authors: Li, Jiwen, Lin, Qiushi, Wang, Weidong, Wade, Paul, Wong, Jiemin
Format: Article
Language:English
Subjects:
Acetylation
Adenosine Triphosphatases
Animals
Autoantigens - metabolism
Blotting, Western
Chromatin - metabolism
Chromatin Immunoprecipitation
DNA Helicases
Histone Deacetylase 1
Histone Deacetylase 2
Histone Deacetylases - chemistry
Histone Deacetylases - metabolism
Ligands
Mi-2 Nucleosome Remodeling and Deacetylase Complex
Mi-2 protein
Models, Biological
N-CoR protein
NURD protein
Plasmids - metabolism
Protein Binding
Repressor Proteins
Research Communication
Saccharomyces cerevisiae Proteins
Sin3 protein
Transcription Factors - metabolism
Transcription, Genetic
Xenopus
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Summary:Specific recruitment of corepressor complexes containing histone deacetylases (HDAC) by transcription factors is believed to play an essential role in transcriptional repression. Recent studies indicate that repression by unliganded nuclear hormone receptors and by the Mad family of repressors requires distinct HDAC-containing corepressor complexes. In this work, we show that unliganded TR specifically recruits only the closely related N-CoR and SMRT-HDAC3 complexes, whereas the Mad1 recruits only the Sin3-HDAC1/2 complex. Significantly, both the Sin3 and Mi-2/NURD complexes also exhibit constitutive association with chromatin and contribute to chromatin deacetylation in a nontargeted fashion. These results suggest that HDAC complexes can contribute to gene repression by two distinct mechanisms as follows: (1) specific targeting by repressors and (2) constitutive association with chromatin.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.962502