Human mixed lymphocyte cultures. Evaluation of a microculture technique utilizing the Multiple Automated Sample Harvester (MASH)

Use of lymphocyte cultures for in vitro studies such as pretransplant histocompatibility testing has established the need for standardization of this technique. A microculture technique has been developed that has facilitated the culturing of lymphocytes and increased the quantity of cultures feasib...

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Bibliographic Details
Published in:Clinical and experimental immunology 1973-10, Vol.15 (2), p.289-302
Main Authors: Thurman, G B, Strong, D M, Ahmed, A, Green, S S, Sell, K W, Hartzman, R J, Bach, F H
Format: Article
Language:English
Subjects:
Automation - instrumentation
Bicarbonates
Buffers
Cell Separation
Cells, Cultured
Centrifugation
Culture Media
Filtration
Humans
Isoantigens
Leukocyte Count
Lymphocyte Culture Test, Mixed
Lymphocytes - immunology
Mitomycins - pharmacology
Thymidine
Tritium
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Summary:Use of lymphocyte cultures for in vitro studies such as pretransplant histocompatibility testing has established the need for standardization of this technique. A microculture technique has been developed that has facilitated the culturing of lymphocytes and increased the quantity of cultures feasible, while lowering the variation between replicate samples. Cultures were prepared for determination of tritiated thymidine incorporation using a Multiple Automated Sample Harvester (MASH). Using this system, the parameters that influence the in vitro responsiveness of human lymphocytes to allogeneic lymphocytes have been investigated.
ISSN:0009-9104
1365-2249