The induction of cell‐associated and secreted IL‐1 by iscoms, matrix or micelles in murine splenic cells

• Published in: Clinical and experimental immunology 1993-07, Vol.93 (1), p.120-125
• Main Authors: VILLACRES‐ERIKSSON, M., BERGSTRÖM‐MOLLAOGLU, M., KÅBERG, H., LÖVGREN, K., MOREIN, B.
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; antigen presentation; Antigen-Presenting Cells - immunology; B-Lymphocytes - immunology; Biological and medical sciences; Cell Line; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Germ-Free Life; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins, Viral - immunology; IL‐1; Immunobiology; Influenza Vaccines - immunology; influenza virus; Interleukin-1 - biosynthesis; Interleukin-1 - immunology; iscom; ISCOMs - immunology; Kinetics; Lymphokines, interleukins ( function, expression); Mice; Mice, Inbred BALB C; Micelles; Quil A; Regulatory factors and their cellular receptors; Spleen - immunology
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.1993.tb06507.x

SUMMARY The kinetics of the expression of membrane‐associated IL‐1 (mIL‐1) and soluble IL‐1 (sIL‐1) was studied in in vitro stimulated spleen cells from non‐primed mice or from mice primed with influenza virus antigens incorporated in the immuno‐stimulating complexes (iscoms) or as micelles. Matrix, which is the carrier structure for the antigens in the iscom, was used as a non‐antigen stimulus. The IL‐1 produced was assayed in an IL‐1‐dependent cell line and the specificity was demonstrated in a blocking experiment with antiserum to IL‐1α. Soluble IL‐1α was also quantified in ELISA. Iscoms and matrix induced production of mIL‐1 and sIL‐1 in cultures from non‐treated mice as well as from mice primed 4 days before with iscoms or micelles. Micelles were a less strong stimulus and did not induce production of sIL‐1. Micelles induced production of mIL‐1 in cultures from non‐primed mice or from mice which were recently immunized with micelles. No mIL‐1 expression was induced by micelles if the spleen cells originated from mice immunized shortly before with iscoms. Depletion experiments demonstrated that sIL‐1 was produced by adherent cells upon stimulation with iscoms or matrix. However, factor(s) from the non‐adherent cells seem to be necessary for optimal secretion of sIL‐1.