Immunogenicity of the meningococcal stress protein MSP63 during natural infection

SUMMARY Acute‐ and convalesccnt‐phasc sera from 40 patients with meningococcal disease were evaluated for immunorcactivity with the meningococcal member of the hsp60 stress protein family. The IgG response was measured by ELISA, using bacterial cell lysate of the corresponding patients' strain,...

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Bibliographic Details
Published in:Clinical and experimental immunology 1993-09, Vol.93 (3), p.377-381
Main Authors: PANNEKOEK, Y., SCHUURMAN, I. G. A., DANKERT, J., PUTTEN, J. P. M.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Antibodies, Bacterial - biosynthesis
Bacterial diseases
Bacterial Proteins - immunology
Biological and medical sciences
Child
Child, Preschool
Escherichia coli
GroEL
Heat-Shock Proteins - immunology
hsp60
Human bacterial diseases
Humans
IgG response
Immunoglobulin G - biosynthesis
Infant
Infectious diseases
Medical sciences
meningococcal disease
Meningococcal Infections - immunology
Middle Aged
Miscellaneous
Mycobacterium
Neisseria
Neisseria meningitidis
Neisseria meningitidis - immunology
Neisseria meningitldis
Staphylococcal infections, streptococcal infections, pneumococcal infections
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Summary:SUMMARY Acute‐ and convalesccnt‐phasc sera from 40 patients with meningococcal disease were evaluated for immunorcactivity with the meningococcal member of the hsp60 stress protein family. The IgG response was measured by ELISA, using bacterial cell lysate of the corresponding patients' strain, and purified hsp60 proteins from Neisseria meningitldis (MSP63), Escherichia coli (GroEL) and Mycobacterium bemis BCG (65K.) as antigens. Analysis of the antibody responses revealed that 24/35 patients (69%) with elevated anti‐meningococcal titres. generated anti‐MSP63 antibodies during the time course of infection. Twelve of these patients generated antibodies specific for MSP63. in six patients anti‐MSP63 levels exceeded anli‐GroEL/65K antibodies. In the remaining six patients, equal levels of anti‐MSP63 and anti‐GroEL/65K were measured. We conclude that MSP63 is expressed and immunogenic during natural meningococcal infection, and that individual subjects have a restricted response to the antigen, resulting in the recognition of Neisseria‐sp hsp60 epitopes and/or cross‐reactive hsp60 determinants.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1993.tb08188.x