Successful primate immunization with peptides conjugated to purified protein derivative or mycobacterial heat shock proteins in the absence of adjuvants

• Published in: Clinical and experimental immunology 1993-09, Vol.93 (3), p.382-386
• Main Authors: PERRAUT, R., LUSSOW, A. R., GAVOILLE, S., GARRAUD, O., MATILE, H., TOLGNE, C., EMBDEN, J., ZEE, R., LAMBERT, P‐H., GYSIN, J., GIUDICE, G.
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - administration & dosage; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Protozoan - blood; Antibody production; BCG Vaccine - immunology; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; heat shock proteins; Heat-Shock Proteins - administration & dosage; Heat-Shock Proteins - immunology; Immunization; Immunobiology; Infection and Immunity; malaria; Male; monkeys; Mycobacterium; Mycobacterium - immunology; Mycobacterium tuberculosis - immunology; Plasmodium falciparum; Plasmodium falciparum - immunology; Primates; Protozoan Proteins - immunology; Protozoan Vaccines - immunology; Saimiri; Saimiri sciureus; synthetic peptides; Tuberculin - administration & dosage; Tuberculin - immunology; vaccines; Vaccines, Synthetic - immunology
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.1993.tb08189.x

SUMMARY We have previously shown in mice that antibodies can be induced to synthetic malaria peptides conjugated to mycobacterial antigens, such as purilied protein derivative (PPD) or heal shock proteins (hsp), and given in the absence of adjuvants after a previous priming with bacille Calmette‐Guerin (BCG). In the present study we investigated this model of immunization in the non‐human primates. Saimiri sciureus monkeys. Monkeys primed with BCG subcutaneously and then immunized subcutaneously with the Plasmodium falciparum sporozoite (NANP)*, synthetic peptide conjugated to PPD or mycobacterial hsp of 65 or 70 kD. in the absence of adjuvants, produced anti‐peptide and anti‐sporozoite IgG anybodies. Interestingly, the carrier effect of the hsp of 70 k D for the induction of anti‐(NANP)40 antibodies was also observed in the absence of a previous priming with BCG. These data suggest that such a vaccination strategy may be applied to humans.