Exposure of the Rh0(D) antigen on the surface and cytoplasmic domains of the red cell membrane

Inside-out (IO) and right-side-out (RO) vesicles derived form human red blood cells were tested for their ability to bind 125I-labelled IgG anti-RHO(D). The binding of anti-RHO(D) to RO vesicles from RHO(D)-positive cells was quantitatively similar to that exhibited by intact cells when compared on...

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Bibliographic Details
Published in:Immunology 1982-01, Vol.45 (1), p.27-30
Main Authors: Kleeman, J E, Masouredis, S P, Victoria, E J
Format: Article
Language:English
Subjects:
Acetylcholinesterase - metabolism
Antigens, Surface - immunology
Cytoplasm - immunology
Erythrocyte Membrane - immunology
Erythrocytes - immunology
Humans
Rh-Hr Blood-Group System - immunology
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Summary:Inside-out (IO) and right-side-out (RO) vesicles derived form human red blood cells were tested for their ability to bind 125I-labelled IgG anti-RHO(D). The binding of anti-RHO(D) to RO vesicles from RHO(D)-positive cells was quantitatively similar to that exhibited by intact cells when compared on a membrane surface area basis. There was no significant binding of labelled antibody to IO vesicles from RhO(D)-positive cells or to either RO or IO vesicles derived from RhO(D)-negative cells. The RhO(D) antigen was immunologically accessible on only the plasma side of the membrane in RhO(D)-positive red cells, as has been shown for blood group antigens defined by carbohydrate determinants. No immunologically reactive RhO(D) antigen was present on either RO or IO vesicles derived from RHO(D)-negative red cells.
ISSN:0019-2805
1365-2567