Generation of chemotactic factor for granulocytes and monocytes from serum by fractions of Brucella abortus

Several fractions isolated from Brucella abortus were examined for their ability to generate chemotactic factor from normal serum. Phenol phase lipopolysaccharides exhibited activity equivalent to that obtained with E. Coli lipopolysaccharide. A carbohydrate-rich aqueous methanol fraction was inhibi...

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Bibliographic Details
Published in:Immunology 1982-05, Vol.46 (1), p.17-22
Main Authors: Birmingham, J.R, Tabatabai, L.B, Deyoe, B.L, Jeska, E.L, Nuessen, M.E
Format: Article
Language:English
Subjects:
animal diseases
animal health
Animals
bacterial infections
Brucella abortus - immunology
Cattle
Chemotactic Factors - immunology
Chemotaxis, Leukocyte
Complement C5 - deficiency
Granulocytes - immunology
Hot Temperature
Lipopolysaccharides - pharmacology
Male
Methanol - pharmacology
Monocytes - immunology
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Summary:Several fractions isolated from Brucella abortus were examined for their ability to generate chemotactic factor from normal serum. Phenol phase lipopolysaccharides exhibited activity equivalent to that obtained with E. Coli lipopolysaccharide. A carbohydrate-rich aqueous methanol fraction was inhibitory at high concentrations, but a non-dialysable component of this fraction contained a potent stimulator of chemotactic activity. Protein-rich fractions from both strain 19 and strain 2308 were inactive. Preheating the serum at 56 degrees for 30 min prevented generation of chemotactic activity by the various fractions, suggesting a role for serum complement. No chemotactic activity was produced by Brucella fractions in C5-deficient DBA/2J mouse serum.
ISSN:0019-2805
1365-2567