Adaptive Responses and Apoptosis in Endothelial Cells Exposed to Carbon Monoxide

• Published in: Proceedings of the National Academy of Sciences - PNAS 2000-02, Vol.97 (3), p.1305-1310
• Main Authors: Thom, Stephen R., Fisher, Donald, Xu, Y. Anne, Notarfrancesco, Kathy, Ischiropoulos, Harry
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Animals; Antioxidants; Apoptosis - drug effects; Biological Sciences; Carbon monoxide; Carbon Monoxide - toxicity; Caspases - physiology; Catalase - metabolism; Cattle; Cell death; Cell growth; Cells; Cells, Cultured; Cellular biology; Endothelial cells; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - enzymology; Enzymes; Ethidium - analogs & derivatives; Ethidium - metabolism; Fluorescence; Glutathione Peroxidase - metabolism; Hydrogen Peroxide - metabolism; Inclusion bodies; Nitrates - metabolism; Nitric Oxide - metabolism; Oxidative Stress; Potassium phosphates; Pulmonary Artery; Selenomethionine - pharmacology; Superoxide Dismutase - metabolism; Superoxides - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.97.3.1305

Prior studies have shown that exposure to carbon monoxide (CO) will elevate the steady-state concentration of nitric oxide (NO) in several cell types and body organs and that some toxic effects of CO are directed toward endothelial cells. Studies reported in this paper were conducted with bovine pulmonary artery endothelial cells exposed to 10 to 100 ppm CO to achieve concentrations between 11 and 110 nM in air-saturated buffer. Exposure to 11 nM CO increased synthesis of manganous superoxide dismutase and conferred resistance against the lethal effects of 110 nM CO. At concentrations of 88 nM CO or more, exposures for 1 h or longer caused cell death that became apparent 18 h after the exposure ceased. Caspase-1 was activated in response to CO, and cell death was inhibited by a caspase-1 inhibitor. Alteration of proteolytic pathways by CO was indicated by the presence of ubiquitin-containing intracellular inclusion bodies. Morphological changes and caspase activation indicated that cell death was an apoptotic process. Cells exposed to 110 nM CO had higher concentrations of manganous superoxide dismutase and heme oxygenase-1 but no changes in glutathione peroxidase, glucose-6-phosphate dehydrogenase, thiols, or catalase. Elevated levels of antioxidant enzymes and apoptosis were inhibited by the nitric oxide synthase inhibitor, S-isopropylisothiourea, and the peroxynitrite scavenger, selenomethionine. These results show that biochemical effects of CO occur at environmentally relevant concentrations, that apoptotic cell death follows exposure to relatively high concentrations of CO, and that these actions of CO are mediated by nitric oxide.