Detection of Oxygen-Derived Radicals in Biological Systems Using Electron Spin Resonance

Oxygen-centered radicals, particularly the hydroxyl and superoxide radicals, have been postulated in many biochemical reactions and have been implicated in many adverse reactions in vivo. This article begins with a review of spin-trapping detection of oxygen-centered radicals in vitro and concludes...

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Bibliographic Details
Published in:Environmental health perspectives 1994-12, Vol.102 (Suppl 10), p.33-36
Main Authors: Mason, Ronald P., Hanna, Phillip M., Burkitt, Mark J., Kadiiska, Maria B.
Format: Article
Language:English
Subjects:
Acute Disease
Adducts
Animals
Bile
Bile - chemistry
Biochemistry
Biomedical materials
Copper - toxicity
Dimethyl Sulfoxide - pharmacology
Electron paramagnetic resonance
Electron spin resonance
Electron Spin Resonance Spectroscopy
Free radicals
Hydrogen
Hydroxyl Radical - analysis
Hydroxyl radicals
In vivo testing
In vivo tests
Iron
Iron - toxicity
Oxygen Radicals: Detection Techniques
Paraquat - pharmacology
Peroxides
Poisoning
Radicals
Reactive Oxygen Species - analysis
Superoxides
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Summary:Oxygen-centered radicals, particularly the hydroxyl and superoxide radicals, have been postulated in many biochemical reactions and have been implicated in many adverse reactions in vivo. This article begins with a review of spin-trapping detection of oxygen-centered radicals in vitro and concludes with a presentation of our approach to the detection of the hydroxyl radicals in models of acute iron and copper poisoning.
ISSN:0091-6765
1552-9924
DOI:10.1289/ehp.94102s1033