The promoter of the gene encoding the C4 form of phosphoenolpyruvate carboxylase directs mesophyll-specific expression in transgenic C4 Flaveria spp

The function of the C4 mechanism of photosynthesis depends on the strict compartmentation of the enzymes involved. Here, we investigate the regulatory mechanisms that ensure the mesophyll-specific expression of the C4 isoform of phosphoenolpyruvate carboxylase. We show that 2 kb of the 5' flank...

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Bibliographic Details
Published in:The Plant cell 1997-04, Vol.9 (4), p.479-489
Main Authors: Stockhaus, J. (Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany.), Schlue, U, Koczor, M, Chitty, J.A, Taylor, W.C, Westhoff, P
Format: Article
Language:English
Subjects:
ADN RECOMBINADO
ADN RECOMBINE
ARN MENSAJERO
ARN MESSAGER
Bundle sheath cells
C4 plants
COMPOSITAE
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
FOSFENOLPIRUVATO CARBOXILASA
GENE
GENES
GLICOSIDASAS
GLYCOSIDASE
HOJAS
Leaves
MESOFILO
Mesophyll cells
MESOPHYLLE
Parenchyma
PHOSPHOENOLPYRUVATE CARBOXYLASE
Plant cells
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
RACINE
RAICES
RNA
SISTEMA VASCULAR DE LA PLANTA
Stems
SYSTEME VASCULAIRE DES PLANTES
TALLO
TIGE
Transgenic plants
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Summary:The function of the C4 mechanism of photosynthesis depends on the strict compartmentation of the enzymes involved. Here, we investigate the regulatory mechanisms that ensure the mesophyll-specific expression of the C4 isoform of phosphoenolpyruvate carboxylase. We show that 2 kb of the 5' flanking region of the Flaveria trinervia C4 PpcA1 gene is sufficient to direct mesophyll-specific expression of the beta-glucuronidase reporter gene in transgenic F. bidentis (C4) plants. In young leaves of seedlings, the activity of this promoter is dependent on the developmental stage of the mesophyll cells. It is induced in a basipetal fashion (leaf tip to base) during leaf development. The promoter region of the orthologous nonphotosynthetic Ppc gene of F. pringlei (C3) induces reporter gene expression mainly in the vascular tissue of leaves and stems as well as in mesophyll cells of transgenic F. bidentis plants. Our experiments demonstrate that during the evolution of the C4 Flaveria species, cis-acting elements of the C4 Ppc gene must have been altered to achieve mesophyll-specific expression
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.9.4.479