Upregulation by glucocorticoids of responses to eosinopoietic cytokines in bone‐marrow from normal and allergic mice

Since the production of eosinopoietic cytokines (GM‐CSF, IL‐3, IL‐5) is inhibited by glucocorticoids, while responsiveness to these cytokines is enhanced in bone‐marrow of allergic mice, we studied the ability of glucocorticoids to modulate murine bone‐marrow eosinopoiesis. Progenitor (semi‐solid) a...

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Bibliographic Details
Published in:British journal of pharmacology 2000-04, Vol.129 (8), p.1543-1552
Main Authors: Gaspar Elsas, Maria Ignez C, Maximiano, Elisabeth S, Joseph, Danielle, Alves, Letícia, Topilko, Andrzej, Vargaftig, B Boris, Xavier Elsas, P
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Anti-Inflammatory Agents - pharmacology
Biological and medical sciences
Bone Marrow - metabolism
Bones, joints and connective tissue. Antiinflammatory agents
bone‐marrow
Cells, Cultured
Cytokines - metabolism
Dexamethasone - pharmacology
eosinophil
Eosinophils - drug effects
Eosinophils - metabolism
Female
Glucocorticoids
Glucocorticoids - metabolism
GM‐CSF
Hypersensitivity - metabolism
IL‐5
Immunization
Male
Medical sciences
Mice
Mice, Inbred BALB C
Pharmacology. Drug treatments
Receptors, Glucocorticoid - drug effects
Receptors, Glucocorticoid - metabolism
Up-Regulation
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Summary:Since the production of eosinopoietic cytokines (GM‐CSF, IL‐3, IL‐5) is inhibited by glucocorticoids, while responsiveness to these cytokines is enhanced in bone‐marrow of allergic mice, we studied the ability of glucocorticoids to modulate murine bone‐marrow eosinopoiesis. Progenitor (semi‐solid) and/or precursor (liquid) cultures were established from bone‐marrow of: (a) normal mice; (b) ovalbumin‐sensitized and challenged mice or (c) dexamethasone (1–5 mg kg−1) injected mice. Cultures were established with GM‐CSF (2 ng ml−1) or IL‐5 (1 ng ml−1), respectively, alone or associated with dexamethasone, hydrocortisone or corticosterone. Total myeloid colony numbers, frequency and size of eosinophil colonies, and numbers of eosinophil‐peroxidase‐positive cells were determined at day 7. In BALB/c mice, dexamethasone (10−7 M) increased GM‐CSF‐stimulated myeloid colony formation (P=0.01), as well as the frequency (P=0.01) and size (P
ISSN:0007-1188
1476-5381
DOI:10.1038/sj.bjp.0703145