Characterization of the effects of cannabinoids on guinea‐pig tracheal smooth muscle tone: role in the modulation of acetylcholine release from parasympathetic nerves

We investigated the ability of the cannabinoid agonists CP55,940 (CB1/CB2) and anandamide (endogenous cannabinoid) to modulate electrical field stimulation (EFS)‐induced acetylcholine (ACh) release from parasympathetic nerve terminals innervating guinea‐pig trachea. We assessed whether modulation of...

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Bibliographic Details
Published in:British journal of pharmacology 2000-08, Vol.130 (7), p.1720-1726
Main Authors: Spicuzza, Lucia, Haddad, El‐Bdaoui, Birrell, Mark, Ling, Andrea, Clarke, Deborah, Venkatesan, Priya, Barnes, Peter J, Belvisi, Maria G
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
acetylcholine release
airways
Analgesics - pharmacology
Analgesics, Non-Narcotic - pharmacology
anandamine
Animals
Arachidonic Acids - pharmacology
Binding, Competitive
Biological and medical sciences
Cannabinoid
Cannabinoids - pharmacology
Carbachol - pharmacology
contraction
Cyclohexanols - pharmacology
Electric Stimulation
Endocannabinoids
Guinea Pigs
In Vitro Techniques
Male
Medical sciences
Muscle Contraction - drug effects
Muscle, Smooth - drug effects
Muscle, Smooth - physiology
neurotransmission
Parasympathetic Nervous System - drug effects
Parasympathetic Nervous System - metabolism
Parasympatholytics - pharmacology
Pharmacology. Drug treatments
Polyunsaturated Alkamides
Respiratory system
trachea
Trachea - cytology
Trachea - drug effects
Trachea - metabolism
Tritium
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Summary:We investigated the ability of the cannabinoid agonists CP55,940 (CB1/CB2) and anandamide (endogenous cannabinoid) to modulate electrical field stimulation (EFS)‐induced acetylcholine (ACh) release from parasympathetic nerve terminals innervating guinea‐pig trachea. We assessed whether modulation of transmitter release translated to an impact on functional responses by investigating the effect of these agents on contractile responses evoked by EFS and ACh. Furthermore, we evaluated the ability of these compounds to elicit bronchodilation in pre‐contracted guinea‐pig tracheal strips. CP55,940 and anandamide significantly inhibited EFS‐evoked ACh release (maximal inhibition of 35.1±2.9% and 33.4±6.4% at 1 μM, P
ISSN:0007-1188
1476-5381
DOI:10.1038/sj.bjp.0703497