Protease‐activated receptor‐2 (PAR‐2) in the rat gastric mucosa: immunolocalization and facilitation of pepsin/pepsinogen secretion

Agonists of protease‐activated receptor‐2 (PAR‐2) trigger neurally mediated mucus secretion accompanied by mucosal cytoprotection in the stomach. The present study immunolocalized PAR‐2 in the rat gastric mucosa and examined if PAR‐2 could modulate pepsin/pepsinogen secretion in rats. PAR‐2‐like imm...

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Bibliographic Details
Published in:British journal of pharmacology 2002-03, Vol.135 (5), p.1292-1296
Main Authors: Kawao, Naoyuki, Sakaguchi, Yuriko, Tagome, Ai, Kuroda, Ryotaro, Nishida, Shozo, Irimajiri, Kiyohiro, Nishikawa, Hiroyuki, Kawai, Kenzo, Hollenberg, Morley D, Kawabata, Atsufumi
Format: Article
Language:English
Subjects:
Animals
Atropine - pharmacology
Biological and medical sciences
Capsaicin - pharmacology
chief cell
Gastric Mucosa - drug effects
Gastric Mucosa - secretion
gastric secretion
Immunohistochemistry
In Vitro Techniques
Male
Medical sciences
NG-Nitroarginine Methyl Ester - pharmacology
Oligopeptides - pharmacology
Omeprazole - pharmacology
pepsin
Pepsin A - secretion
Pepsinogen A - secretion
Protease‐activated receptor‐2 (PAR‐2)
Rats
Rats, Wistar
Receptor, PAR-2
Receptors, Thrombin - agonists
Receptors, Thrombin - physiology
Stomach - drug effects
Stomach - physiology
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Summary:Agonists of protease‐activated receptor‐2 (PAR‐2) trigger neurally mediated mucus secretion accompanied by mucosal cytoprotection in the stomach. The present study immunolocalized PAR‐2 in the rat gastric mucosa and examined if PAR‐2 could modulate pepsin/pepsinogen secretion in rats. PAR‐2‐like immunoreactivity was abundant in the deep regions of gastric mucosa, especially in chief cells. The PAR‐2 agonist SLIGRL‐NH2, but not the control peptide LSIGRL‐NH2, administered i.v. repeatedly at 0.3 – 1 μmol kg−1, four times in total, significantly facilitated gastric pepsin secretion, although a single dose produced no significant effect. The PAR‐2‐mediated gastric pepsin secretion was resistant to omeprazole, NG‐nitro‐L‐arginine methyl ester (L‐NAME) or atropine, and also to ablation of sensory neurons by capsaicin. Our study thus provides novel evidence that PAR‐2 is localized in mucosal chief cells and facilitates gastric pepsin secretion in the rats, most probably by a direct mechanism. British Journal of Pharmacology (2002) 135, 1292–1296; doi:10.1038/sj.bjp.0704562
ISSN:0007-1188
1476-5381
DOI:10.1038/sj.bjp.0704562