Two monoclonal antibodies raised against a Burkitt lymphoma cell line recognise different cell types within lymphoid follicles

Monoclonal antibodies were raised against a laboratory derived variant of the Raji Burkitt lymphoma cell line. We have characterized two of these antibodies by screening on haematopoietic cell lines, and frozen sections of both reactive and neoplastic lymphoid tissue, and found reactivity with separ...

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Bibliographic Details
Published in:Clinical and experimental immunology 1985-02, Vol.59 (2), p.315-326
Main Authors: MURRAY, L. J, DHUT, S, WALKER, L. C, RAINEY, M, HABESHAW, J. A
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
B-Lymphocytes - immunology
Biological and medical sciences
Burkitt Lymphoma - immunology
Cell Line
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Leukemia, Hairy Cell - immunology
Leukemia, Lymphoid - immunology
Lymphocyte Activation
Lymphoid Tissue - immunology
Lymphoma - immunology
Male
Mice
Mice, Inbred BALB C
Organs and cells involved in the immune response
Phenotype
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Summary:Monoclonal antibodies were raised against a laboratory derived variant of the Raji Burkitt lymphoma cell line. We have characterized two of these antibodies by screening on haematopoietic cell lines, and frozen sections of both reactive and neoplastic lymphoid tissue, and found reactivity with separate cellular components of lymphoid follicles. Monoclonal FW37.4.D5 reacts in section specifically with follicular dendritic reticular cells. Monoclonal 35.1C5 reacts with a subpopulation of splenic marginal zone, and lymph node mantle zone, B lymphocytes, but stains only rare cells within germinal centres. This monoclonal is operationally B lymphocyte specific, distinguishing an 'intermediate' B cell subset, represented in lymphoma by B chronic lymphocytic leukaemia, B hairy cell leukaemia and centrocytic lymphoma.
ISSN:0009-9104
1365-2249