The distribution and molecular presentation of the brush border antigen of Heymann nephritis in various rat tissues

A monospecific antiserum to gp600, a recently isolated and characterized antigen of Heymann nephritis (HN), was used in radioimmunoassay to measure the specific activity of the antigen in various rat tissues. Lung contained the highest amount followed by pancreas, stomach, small intestine, kidney, l...

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Bibliographic Details
Published in:Clinical and experimental immunology 1985-06, Vol.60 (3), p.579-585
Main Authors: SINGH, A. K, MAKKER, S. P
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity
Antigens - analysis
Binding, Competitive
Biological and medical sciences
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Glomerulonephritis
Lung - immunology
Male
Medical sciences
Microvilli - immunology
Molecular Weight
Nephritis - immunology
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Pancreas - immunology
Rabbits
Radioimmunoassay
Rats
Rats, Inbred Lew
Tissue Distribution
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Summary:A monospecific antiserum to gp600, a recently isolated and characterized antigen of Heymann nephritis (HN), was used in radioimmunoassay to measure the specific activity of the antigen in various rat tissues. Lung contained the highest amount followed by pancreas, stomach, small intestine, kidney, large intestine, seminal vesicle, epididymis, muscle, heart, brain, spleen, liver, testis and serum. Immunoprecipitation of the antigen with anti-gp600 and analyses of the precipitates on SDS-PAGE revealed that the majority of the tissues contained a cross-reactive 90 kD polypeptide antigen. Four of these tissues also contained a 66kD polypeptide antigen. Seminal vesicles, brain, kidney and liver contained a high mol. wt (greater than 330kD) antigen. Kidney possessed a unique set of antigens, namely 330kD, 110kD and triplet of 80kD besides the common 90kD and greater than 330kD antigens. These results show that antigens cross-reactive with HN gp600 antigen are present in other tissues but the biochemical nature of the kidney antigen is unique in comparison to antigen present in other tissues.
ISSN:0009-9104
1365-2249