Effect of salt stress on germin gene expression in barley roots

• Published in: Plant physiology (Bethesda) 1996-03, Vol.110 (3), p.971-977
• Main Authors: Hurkman, W.J. (USDA, ARS, Western Regional Research Center, Crop Improvement and Utilization Research Unit, Albany, CA.), Tanaka, C.K
• Format: Article
• Language: English
• Subjects: ABA; ABSORCION; ABSORPTION; Agronomy. Soil science and plant productions; AIA; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; ARN MENSAJERO; ARN MESSAGER; Barley; Biological and medical sciences; CHLORURE DE SODIUM; CHOIX DE LA DATE; CLORURO SODICO; Complementary DNA; Economic plant physiology; ELECCION DE LA EPOCA; ETAPAS DE DESARROLLO; ETAPAS DE DESARROLLO DE LA PLANTA; EXPRESION GENICA; EXPRESSION DES GENES; FACTEUR DU MILIEU; FACTORES AMBIENTALES; Fundamental and applied biological sciences. Psychology; Gene expression; GERMINACION; GERMINATION; HOMEOSTASIE; HOMEOSTASIS; HORDEUM VULGARE; INHIBIDORES DEL CRECIMIENTO; INHIBITEUR DE CROISSANCE; Messenger RNA; Molecular and cellular biology; Molecular genetics; OXALATE; Oxalates; OXALATOS; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plant physiology and development; Plant roots; Plants; PLANTULAS; PLANTULE; RACINE; RAICES; RNA; SALICILATOS; SALICYLATE; SALINIDAD; SALINITE; Seedlings; STADE DE DEVELOPPEMENT; STADE DE DEVELOPPEMENT VEGETAL; Table salt; TALLO; TIGE; Water and solutes. Absorption, translocation and permeability; Water relations, transpiration, stomata; Whole Plant, Environmental, and Stress Physiology
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.110.3.971

Germin gene expression in barley (Hordeum vulgare L.) seedlings responds to developmental and environmental cues. During seed germination, germin mRNA levels were maximal 2 d after the start of imbibition in control seedlings and declined to low levels by 6 d. When seeds were sown in the presence of 200 mM NaCl, germin mRNA levels were also maximal after 2 d, but NaCl treatment, which slowed seedling growth, prolonged germin gene expression for an additional 1 d. In 4-d-old seedlings, germin mRNA levels were highest in roots and higher in the vascular transition region than in shoots. In roots of 6-d-old seedlings, germin gene expression was regulated by salt shock and plant growth regulators. Induced germin mRNA levels were maximal 8 h after treatment with NaCl, salicylate, methyl salicylate, or methyl jasmonate and 4 h after treatment with abscisic acid and indoleacetic acid. Like germin mRNA, dehydrin mRNA levels were maximal 8 h after NaCl treatment. In contrast, peroxidase mRNA levels declined to less than control levels within 30 min of treatment. Germin gene expression is regulated developmentally by salt stress and by treatments with plant hormones. Since germin is an oxalate oxidase, these results imply that oxalate has important roles in plant development and homeostasis