Differential expression and turnover of the tomato polyphenol oxidase gene family during vegetative and reproductive development

Polyphenol oxidases (PPOs) are encoded by a highly conserved, seven-member gene family clustered within a 165-kb locus on chromosome 8 of tomato (Lycopersicon esculentum). Using gene-specific probes capable of differentiating between PPO A/C, PPO B, PPO D, and PPO E/F, we examined the spatial and te...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1997-03, Vol.113 (3), p.707-718
Main Authors: Thipyapong, P. (Cornell University, Ithaca, NY.), Joel, D.M, Steffens, J.C
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
Biological and medical sciences
CATECHOL OXYDASE
CATECOL OXIDASA
CHIMIE
Enzymes
Epidermal cells
Epidermis
ETAPAS DE DESARROLLO
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
FLEUR
FLORES
FRUITS
FRUTAS
Fundamental and applied biological sciences. Psychology
GENE
Gene Regulation and Molecular Genetics
GENES
HOJAS
LYCOPERSICON ESCULENTUM
Magnification
Metabolism
Mother cells
Ovules
Oxidases
PERIODE VEGETATIVE
PERIODO VEGETATIVO
Plant physiology and development
Plants
Pollen
Polyphenols
QUIMICA
RACINE
RAICES
REPRODUCCION
REPRODUCTION
STADE DE DEVELOPPEMENT
STADE DE DEVELOPPEMENT VEGETAL
TALLO
TIGE
Trichomes
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Summary:Polyphenol oxidases (PPOs) are encoded by a highly conserved, seven-member gene family clustered within a 165-kb locus on chromosome 8 of tomato (Lycopersicon esculentum). Using gene-specific probes capable of differentiating between PPO A/C, PPO B, PPO D, and PPO E/F, we examined the spatial and temporal expression of this gene family during vegetative and reproductive development. RNA blots and in situ hybridization using these probes showed that although PPO expression is primarily confined to early stages of development, the steady-state mRNA levels of these genes are sublect to complex patterns of spatial and temporal regulation in vegetative and reproductive organs. Young tomato leaves and flowers possess the most abundant PPO transcripts. PPO B is the most abundant in young leaves, whereas in the inflorescence PPO B and E/F transcripts are dominant. Differential expression of PPOs is also observed in various trichome types. PPO A/C are specifically expressed in type I and type IV trichomes. In contrast, PPO D is only expressed in type VI trichomes. Type I, IV, and VI trichomes possess PPO E/F transcripts. Immunolocalization verified the translational activity of PPOs identified by in situ hybridization and suggested cell-type-specific, developmentally programmed PPO turnover. In addition, immunolocalization demonstrated the accumulation of PPO in specific idioblast cells of stems, leaves, and fruits
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.113.3.707