Effects of a mechanical stimulation on localization of annexin-like proteins in Bryonia dioica internodes

• Published in: Plant physiology (Bethesda) 1997-07, Vol.114 (3), p.981-988
• Main Authors: Thonat, C, Mathieu, C, Crevecoeur, M, Penel, C, Gaspar, T, Boyer, N
• Format: Article
• Language: English
• Subjects: ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; Animals; Annexins; Annexins - biosynthesis; Annexins - isolation & purification; Antibodies; Antiserum; BINDING PROTEINS; Biological and medical sciences; Blotting, Western; BRYONIA CRETICA; Calcium - metabolism; Cell Biology and Signal Transduction; CELL MEMBRANES; CHEMICAL COMPOSITION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CUCURBITACEAE; Electrophoresis; Electrophoresis, Polyacrylamide Gel; ENTRE NOEUD; ESTIMULO; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; Gels; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; IMMUNOLOGIE; IMMUNOLOGY; INMUNOFLUORESCENCIA; INMUNOLOGIA; INTERNODES; INTERNODIOS; Intracellular Membranes - metabolism; MECHANICAL METHODS; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; METHODE MECANIQUE; METODOS MECANICOS; Microsomes - metabolism; PARENCHYMA; PARENCHYME; PARENQUIMA; PHOSPHATIDE; PHOSPHOLIPIDS; Physical agents; Physical Stimulation; PLANT ANATOMY; Plant Physiological Phenomena; Plant physiology and development; Plant Proteins - biosynthesis; Plant Proteins - isolation & purification; Plants; PLASMA MEMBRANES; PROTEIN SYNTHESIS; PROTEINAS; PROTEINAS AGLUTINANTES; PROTEINE; PROTEINE DE LIAISON; PROTEINS; PURIFICACION; PURIFICATION; Rabbits; SINTESIS DE PROTEINAS; STIMULI; STIMULUS; STRESS RESPONSE; SYNTHESE PROTEIQUE; Vegetative apparatus, growth and morphogenesis. Senescence
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.114.3.981

Mechanical stimulation exerted by rubbing a young internode of Bryonia dioica plants inhibits its growth. Previous cellular and biochemical studies showed that this growth inhibition is associated with Ca2+ redistribution and profound modifications of plasma membrane characteristics. We extracted and purified Ca2+-dependent phospholipid-binding proteins from B. dioica internodes. Two main proteins, p33 and p35, and other minor bands were isolated and identified as annexin-like proteins because of their biochemical properties and their cross-reactions with antibodies against maize (Zea mays L.) annexing. Rabbit antiserum was obtained by injection of B. dioica p35. This antiserum was used for the immunocytolocalization of annexin-like proteins in internode parenchyma cells. It appeared that the distribution of annexin-like proteins was different before and 30 min after the mechanical stimulation. Western analysis of proteins in membrane fractions after separation by free-flow electrophoresis showed that p35 was present in most fractions, whereas p33 appeared mainly in plasmalemma-enriched fractions after the mechanical stimulation. It is hypothesized that a subcellular redistribution of these proteins might be involved in growth inhibition by mechanical stress