Diverse range of small peptides associated with high-density lipoprotein

High-density lipoproteins (HDL) were examined as potential carriers of small peptides in plasma. HDL purified by density gradient centrifugation was delipidated and fractionated by size-exclusion chromatography under denaturing conditions. By HPLC and mass spectrometry, more than 100 peptide compone...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-02, Vol.340 (3), p.909-915
Main Authors: Hortin, Glen L., Shen, Rong-Fong, Martin, Brian M., Remaley, Alan T.
Format: Article
Language:English
Subjects:
alpha 1-Antitrypsin - chemistry
Apolipoprotein
Apolipoprotein A-I
Apolipoproteins - chemistry
Blood Proteins - chemistry
Calibration
Centrifugation, Density Gradient
Chromatography
Chromatography, High Pressure Liquid
Fibrinogen - chemistry
High-density lipoprotein
Humans
Lipoproteins, HDL - chemistry
Mass Spectrometry
Peptides - chemistry
Peptidomics
Prealbumin - chemistry
Proteomics
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Transthyretin
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Summary:High-density lipoproteins (HDL) were examined as potential carriers of small peptides in plasma. HDL purified by density gradient centrifugation was delipidated and fractionated by size-exclusion chromatography under denaturing conditions. By HPLC and mass spectrometry, more than 100 peptide components were found in the size range from 1000 to 5000 Da. By sequence analysis, peptides were identified as fragments of proteins such as apolipoproteins, fibrinogen, α 1-proteinase inhibitor, and transthyretin. The results indicate that purified HDL bears a complex range of small peptides. It is unclear whether the peptides have any significant functional role as apolipopeptides, but they may represent a pathway for peptide delivery or scavenging and a significant reservoir of plasma peptides for diagnostic evaluation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.12.098