Comparative studies on ferredoxin-NADP+ oxidoreductase isoenzymes derived from different organs by antibodies specific for the radish root- and leaf-enzymes

Determination of the prosthetic group and titration of sulfhydryl group of ferredoxin-NADP(+) oxidoreductase (FNR) from roots of radish (Raphanus sativus var acanthiformis cv Miyashige) confirmed its similarity to leaf-FNR. Antisera directed against radish root-FNR and leaf-FNR distinguished the enz...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1993-10, Vol.103 (2), p.435-440
Main Authors: Morigasaki, S, Jin, T, Wada, K
Format: Article
Language:English
Subjects:
ACIDE AMINE
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
AMINOACIDOS
Antiserum
Biological and medical sciences
BRYOPHYTA
Cell culture techniques
CODE GENETIQUE
CODIGO GENETICO
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CULTIVO DE CELULAS
CULTURE DE CELLULE
Cultured cells
CYANOPHYTA
Enzymes
FEUILLE
Fundamental and applied biological sciences. Psychology
GENETICA
GENETIQUE
GLYCINE MAX
HOJAS
Horsetails
ISOENZIMAS
ISOENZYME
JAPON
Liverworts
Metabolism
Metabolism and Enzymology
METALLOPROTEINE
METALPROTEINAS
OXIDORREDUCTASAS
OXYDOREDUCTASE
PISUM SATIVUM
Plant physiology and development
Plant roots
Plants
PROPIEDADES FISICO-QUIMICAS
PROPRIETE PHYSICOCHIMIQUE
PROTEINAS
PROTEINE
PTERIDOPHYTA
PURIFICACION
PURIFICATION
RACINE
Radishes
RAICES
RAPHANUS SATIVUS
Spinach
TALLO
TIGE
ZEA MAYS
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Summary:Determination of the prosthetic group and titration of sulfhydryl group of ferredoxin-NADP(+) oxidoreductase (FNR) from roots of radish (Raphanus sativus var acanthiformis cv Miyashige) confirmed its similarity to leaf-FNR. Antisera directed against radish root-FNR and leaf-FNR distinguished the enzyme forms from roots and leaves of radish as well as other flowering plants. The FNR isoenzymes showed organ-specific distributions. in horsetail (Equisetum arvense L.) and cultured liverwort cells (Marchantia polymorpha), at least two FNR isoenzymes were distinguished by the antisera. FNR from Chlorella vulgaris reacted only with the anti-root-FNR antiserum. FNR from a cyanobacterium, Spirulina spp., failed to react with either antiserum
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.103.2.435