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Expression and localization of plant protein disulfide isomerase

A cDNA clone encoding a putative protein disulfide isomerase (PDI, EC 5.3.4.1) from alfalfa (Medicago sativa L.) was expressed in Escherichia coli cells, and an antiserum was raised against the expressed PDI-active protein. The antiserum recognized a protein of approximately 60 kD in extracts from a...

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Published in:	Plant physiology (Bethesda) 1993-11, Vol.103 (3), p.719-726
Main Authors:	Shorrosh, B.S, Subramaniam, J, Schubert, K.R, Dixon, R.A
Format:	Article
Language:	English
Subjects:	ADN Alfalfa Analytical, structural and metabolic biochemistry Antiserum Biological and medical sciences Cell walls CELLULE CELULAS CHAMBRE DE CROISSANCE CLONE CLONES CONTROL AMBIENTAL CONTROLE DU MILIEU Cultured cells Disulfides Enzymes Enzymes and enzyme inhibitors ESCHERICHIA COLI FITOTRONES Fundamental and applied biological sciences. Psychology Gels GLYCINE MAX IMMUNSERUM ISOMERASAS ISOMERASE Isomerases MEDICAGO SATIVA Metabolism Metabolism and Enzymology NICOTIANA TABACUM Plant physiology and development Plant roots Plants PROTEINAS PROTEINE Proteins Soybeans SUERO INMUNE SULFURE SULFUROS
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creator	Shorrosh, B.S Subramaniam, J Schubert, K.R Dixon, R.A
description	A cDNA clone encoding a putative protein disulfide isomerase (PDI, EC 5.3.4.1) from alfalfa (Medicago sativa L.) was expressed in Escherichia coli cells, and an antiserum was raised against the expressed PDI-active protein. The antiserum recognized a protein of approximately 60 kD in extracts from alfalfa, soybean, and tobacco roots and stems. Levels of this protein remained relatively constant on exposure of alfalfa cell suspension cultures to the protein glycosylation inhibitor tunicamycin, whereas a slightly lower molecular mass form, also detected by the antiserum, was induced by this treatment. A lower molecular mass form of PDI was also observed in roots of alfalfa seedlings during the first 5 weeks after germination. PDI levels increased in developing soybean seeds up to 17 d after fertilization and then declined. Tissue print immunoblots revealed highest levels of PDI protein in the cambial tissues of soybean stems and petioles and in epidermal, subepidermal, cortical, and pith tissues of stems of alfalfa and tobacco. Immunogold electron microscopy confirmed the localization of PDI to the endoplasmic reticulum in soybean root nodules
doi_str_mv	10.1104/pp.103.3.719
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Immunogold electron microscopy confirmed the localization of PDI to the endoplasmic reticulum in soybean root nodules</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.103.3.719</identifier><identifier>PMID: 12231974</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ADN ; Alfalfa ; Analytical, structural and metabolic biochemistry ; Antiserum ; Biological and medical sciences ; Cell walls ; CELLULE ; CELULAS ; CHAMBRE DE CROISSANCE ; CLONE ; CLONES ; CONTROL AMBIENTAL ; CONTROLE DU MILIEU ; Cultured cells ; Disulfides ; Enzymes ; Enzymes and enzyme inhibitors ; ESCHERICHIA COLI ; FITOTRONES ; Fundamental and applied biological sciences. Psychology ; Gels ; GLYCINE MAX ; IMMUNSERUM ; ISOMERASAS ; ISOMERASE ; Isomerases ; MEDICAGO SATIVA ; Metabolism ; Metabolism and Enzymology ; NICOTIANA TABACUM ; Plant physiology and development ; Plant roots ; Plants ; PROTEINAS ; PROTEINE ; Proteins ; Soybeans ; SUERO INMUNE ; SULFURE ; SULFUROS</subject><ispartof>Plant physiology (Bethesda), 1993-11, Vol.103 (3), p.719-726</ispartof><rights>Copyright 1993 American Society of Plant Physiologists</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-31c18ff922c275db02218dad4812e100d70b9bfbc202f309a39862926a3024713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4275455$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4275455$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,58237,58470</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3929197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12231974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shorrosh, B.S</creatorcontrib><creatorcontrib>Subramaniam, J</creatorcontrib><creatorcontrib>Schubert, K.R</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><title>Expression and localization of plant protein disulfide isomerase</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A cDNA clone encoding a putative protein disulfide isomerase (PDI, EC 5.3.4.1) from alfalfa (Medicago sativa L.) was expressed in Escherichia coli cells, and an antiserum was raised against the expressed PDI-active protein. The antiserum recognized a protein of approximately 60 kD in extracts from alfalfa, soybean, and tobacco roots and stems. Levels of this protein remained relatively constant on exposure of alfalfa cell suspension cultures to the protein glycosylation inhibitor tunicamycin, whereas a slightly lower molecular mass form, also detected by the antiserum, was induced by this treatment. A lower molecular mass form of PDI was also observed in roots of alfalfa seedlings during the first 5 weeks after germination. PDI levels increased in developing soybean seeds up to 17 d after fertilization and then declined. Tissue print immunoblots revealed highest levels of PDI protein in the cambial tissues of soybean stems and petioles and in epidermal, subepidermal, cortical, and pith tissues of stems of alfalfa and tobacco. Immunogold electron microscopy confirmed the localization of PDI to the endoplasmic reticulum in soybean root nodules</description><subject>ADN</subject><subject>Alfalfa</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antiserum</subject><subject>Biological and medical sciences</subject><subject>Cell walls</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CHAMBRE DE CROISSANCE</subject><subject>CLONE</subject><subject>CLONES</subject><subject>CONTROL AMBIENTAL</subject><subject>CONTROLE DU MILIEU</subject><subject>Cultured cells</subject><subject>Disulfides</subject><subject>Enzymes</subject><subject>Enzymes and enzyme inhibitors</subject><subject>ESCHERICHIA COLI</subject><subject>FITOTRONES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>GLYCINE MAX</subject><subject>IMMUNSERUM</subject><subject>ISOMERASAS</subject><subject>ISOMERASE</subject><subject>Isomerases</subject><subject>MEDICAGO SATIVA</subject><subject>Metabolism</subject><subject>Metabolism and Enzymology</subject><subject>NICOTIANA TABACUM</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plants</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>Soybeans</subject><subject>SUERO INMUNE</subject><subject>SULFURE</subject><subject>SULFUROS</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNpVkEFvEzEQRi0EImnhxgkhtIceODRhxvbu2odKoKi0SJU4QM-W12sHR5u1sTdV6a_HUaIAJ4_8vZmxHyFvEJaIwD_GuERgS7ZsUT4jc6wZXdCai-dkDlBqEELOyFnOGwBAhvwlmSGlDGXL5-TT9WNMNmcfxkqPfTUEowf_pKf9RXBVHPQ4VTGFyfqx6n3eDc73tvI5bG3S2b4iL5wesn19PM_J_ZfrH6vbxd23m6-rz3cLw0UzLRgaFM5JSg1t674DSlH0uucCqUWAvoVOdq4zFKhjIDWToqGSNpoB5S2yc3J1mBt33db2xo5T0oOKyW91-q2C9ur_ZPQ_1To8KKwl8H3_h2N_Cr92Nk9q67OxQ_mfDbusUNSStU3byoJeHlCTQs7JutMWBLV3rmIsJVNMFecFf__vy_7CR8kFuDgCOhe7LunR-HzimKSycAV7d8A2eQrpFPPii9d1id8eYqeD0utUJtx_l5w3QjTsD7fQm1c</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Shorrosh, B.S</creator><creator>Subramaniam, J</creator><creator>Schubert, K.R</creator><creator>Dixon, R.A</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19931101</creationdate><title>Expression and localization of plant protein disulfide isomerase</title><author>Shorrosh, B.S ; Subramaniam, J ; Schubert, K.R ; Dixon, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-31c18ff922c275db02218dad4812e100d70b9bfbc202f309a39862926a3024713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ADN</topic><topic>Alfalfa</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antiserum</topic><topic>Biological and medical sciences</topic><topic>Cell walls</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CHAMBRE DE CROISSANCE</topic><topic>CLONE</topic><topic>CLONES</topic><topic>CONTROL AMBIENTAL</topic><topic>CONTROLE DU MILIEU</topic><topic>Cultured cells</topic><topic>Disulfides</topic><topic>Enzymes</topic><topic>Enzymes and enzyme inhibitors</topic><topic>ESCHERICHIA COLI</topic><topic>FITOTRONES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>GLYCINE MAX</topic><topic>IMMUNSERUM</topic><topic>ISOMERASAS</topic><topic>ISOMERASE</topic><topic>Isomerases</topic><topic>MEDICAGO SATIVA</topic><topic>Metabolism</topic><topic>Metabolism and Enzymology</topic><topic>NICOTIANA TABACUM</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plants</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>Soybeans</topic><topic>SUERO INMUNE</topic><topic>SULFURE</topic><topic>SULFUROS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shorrosh, B.S</creatorcontrib><creatorcontrib>Subramaniam, J</creatorcontrib><creatorcontrib>Schubert, K.R</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shorrosh, B.S</au><au>Subramaniam, J</au><au>Schubert, K.R</au><au>Dixon, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and localization of plant protein disulfide isomerase</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>103</volume><issue>3</issue><spage>719</spage><epage>726</epage><pages>719-726</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A cDNA clone encoding a putative protein disulfide isomerase (PDI, EC 5.3.4.1) from alfalfa (Medicago sativa L.) was expressed in Escherichia coli cells, and an antiserum was raised against the expressed PDI-active protein. The antiserum recognized a protein of approximately 60 kD in extracts from alfalfa, soybean, and tobacco roots and stems. Levels of this protein remained relatively constant on exposure of alfalfa cell suspension cultures to the protein glycosylation inhibitor tunicamycin, whereas a slightly lower molecular mass form, also detected by the antiserum, was induced by this treatment. A lower molecular mass form of PDI was also observed in roots of alfalfa seedlings during the first 5 weeks after germination. PDI levels increased in developing soybean seeds up to 17 d after fertilization and then declined. Tissue print immunoblots revealed highest levels of PDI protein in the cambial tissues of soybean stems and petioles and in epidermal, subepidermal, cortical, and pith tissues of stems of alfalfa and tobacco. Immunogold electron microscopy confirmed the localization of PDI to the endoplasmic reticulum in soybean root nodules</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>12231974</pmid><doi>10.1104/pp.103.3.719</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects	ADN Alfalfa Analytical, structural and metabolic biochemistry Antiserum Biological and medical sciences Cell walls CELLULE CELULAS CHAMBRE DE CROISSANCE CLONE CLONES CONTROL AMBIENTAL CONTROLE DU MILIEU Cultured cells Disulfides Enzymes Enzymes and enzyme inhibitors ESCHERICHIA COLI FITOTRONES Fundamental and applied biological sciences. Psychology Gels GLYCINE MAX IMMUNSERUM ISOMERASAS ISOMERASE Isomerases MEDICAGO SATIVA Metabolism Metabolism and Enzymology NICOTIANA TABACUM Plant physiology and development Plant roots Plants PROTEINAS PROTEINE Proteins Soybeans SUERO INMUNE SULFURE SULFUROS
title	Expression and localization of plant protein disulfide isomerase
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