Ethylene-induced increase in glutamine synthetase activity and mRNA levels in Hevea brasiliensis latex cells

Ethylene, used as a stimulant of latex production in Hevea brasiliensis, significantly activates the regenerating metabolism within the laticiferous cells. In this context, attention was focused on glutamine synthetase (GS; EC 6.3.1.2), a key enzyme in nitrogen metabolism. A specific and significant...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1994-05, Vol.105 (1), p.127-132
Main Authors: Pujade-Renaud, V, Clement, A, Perrot-Rechenmann, C, Prevot, J.C, Chrestin, H, Jacob, J.L, Guern, J
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Agronomy. Soil science and plant productions
ARN MENSAJERO
ARN MESSAGER
Biological and medical sciences
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CONTENIDO PROTEICO
Cytosol
Enzymes
ETHYLENE
ETILENO
Fundamental and applied biological sciences. Psychology
Gene expression regulation
GENETICA
GENETIQUE
GENOTIPOS
GENOTYPE
Genotypes
HEVEA BRASILIENSIS
LATEX
LIGASAS
LIGASE
Messenger RNA
Metabolism
Molecular Biology and Gene Regulation
Nitrogen
Plant physiology and development
Protein synthesis
RNA
Rubber
SINTESIS DE PROTEINAS
Storage and secretion, pigments, phytochrome
SYNTHESE PROTEIQUE
TENEUR EN PROTEINES
VARIACION GENETICA
VARIATION GENETIQUE
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Summary:Ethylene, used as a stimulant of latex production in Hevea brasiliensis, significantly activates the regenerating metabolism within the laticiferous cells. In this context, attention was focused on glutamine synthetase (GS; EC 6.3.1.2), a key enzyme in nitrogen metabolism. A specific and significant activation of the cytosolic glutamine synthetase (GScyt) in the laticiferous cells after ethylene treatment parallels the increase of latex yield. A marked accumulation of the corresponding mRNA was found, but in contrast, a slight and variable increase of the polypeptide level is at the limit of detection by western blotting. The GS response to ethylene might be mediated by ammonia that increases in latex cytosol following ethylene treatment. The physiological significance for such a regulation by ethylene of the GScyt is discussed in terms of the nitrogen requirement for protein synthesis associated with latex regeneration
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.105.1.127