Arabidopsis thaliana expresses three divergent Srp54 genes

The Arabidopsis thaliana Srp54 gene family was determined to consist of three genes, all of which were cloned and sequenced. In addition, cDNAs corresponding to two of the genes were obtained. To our knowledge this is the first description of multiple Srp54 genes within an organism. In contrast to t...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1994-11, Vol.106 (3), p.1157-1162
Main Authors: Chu, B. (Rutgers University, New Brunswick, NJ.), Lindstrom, J.T, Belanger, F.C
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Amino acids
Animals
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins
ARABIDOPSIS THALIANA
Biological and medical sciences
CLONE
CLONES
Cloning, Molecular
Complementary DNA
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DNA
DNA probes
DNA, Complementary - isolation & purification
Exons
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
Gels
GENE
Gene expression
GENES
Genes, Plant
Genetic Variation
GENETICA
GENETIQUE
Genomics
GTP-Binding Proteins - biosynthesis
GTP-Binding Proteins - genetics
Introns
Mice
Molecular and cellular biology
Molecular Biology and Gene Regulation
Molecular genetics
Molecular Sequence Data
Multigene Family
Polymerase chain reaction
RNA
Saccharomyces cerevisiae
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
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Description
Summary:The Arabidopsis thaliana Srp54 gene family was determined to consist of three genes, all of which were cloned and sequenced. In addition, cDNAs corresponding to two of the genes were obtained. To our knowledge this is the first description of multiple Srp54 genes within an organism. In contrast to the situation in mammals, where there are only three amino acid differences between the mouse and canine sequences, there was significant amino acid sequence diversity among the genes, particularly in the methionine-rich region of the protein, which is the region responsible for binding to the 7S RNA of the signal recognition particle and to the signal sequence of newly synthesized proteins. The amino acid sequences of the GTP-binding domains of the three clones were 86% identical, whereas the methionine-rich domains were only 65% identical. RNA gel blots of various tissues and developmentai stages hybridized with gene-specific probes revealed that all three genes were expressed in all the tissues investigated. There were, however, quantitative differences in expression levels
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.106.3.1157