Novel regulation of heat shock genes during carrot somatic embryo development

We have determined that somatic embryos of carrot exhibit a number of interesting and unusual properties when exposed to heat shock at different times in their development. Specifically, we have seen that mid-globular embryos can be arrested irreversibly in their development when heat-shocked, where...

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Bibliographic Details
Published in:The Plant cell 1989-12, Vol.1 (12), p.1137-1146
Main Authors: Zimmerman, J.L. (University of Maryland Baltimore County, Catonsville, MD), Apuya, N, Darwish, K, O'Carroll, C
Format: Article
Language:English
Subjects:
AGOTAMIENTO POR EL CALOR
Animals
ARN MENSAJERO
ARN MESSAGER
Callus
Cell lines
COUP DE CHALEUR
CULTIVO IN VITRO
CULTURE IN VITRO
DAUCUS CAROTA
ELECTROFORESIS
ELECTROPHORESE
EMBRIONES VEGETALES
EMBRYON VEGETAL
Embryonic cells
Embryonic Development
Embryos
EXPRESION GENICA
EXPRESSION DES GENES
GENE
Gene Expression Regulation
GENES
GENETICA
GENETIQUE
Heat shock response
Heat-Shock Proteins - biosynthesis
Heat-Shock Proteins - genetics
Hot Temperature
Messenger RNA
Plant Proteins - genetics
PROTEINAS
PROTEINE
RNA
Shock heating
Somatic embryos
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Transcription, Genetic
Vegetables - embryology
Vegetables - genetics
VIABILIDAD
VIABILITE
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Summary:We have determined that somatic embryos of carrot exhibit a number of interesting and unusual properties when exposed to heat shock at different times in their development. Specifically, we have seen that mid-globular embryos can be arrested irreversibly in their development when heat-shocked, whereas all other stages of embryogenesis, both before and after this stage, are fully capable of normal development after the stress. In investigating the molecular basis of this developmental sensitivity to heat shock, using a cloned heat shock gone encoding a small heat shock protein, we have determined that globular embryos both synthesize and accumulate significantly less heat shock mRNA when compared with embryos of any other stage or to callus suspension cells. In fact, there appears to be no transcriptional induction of heat shock gene expression in response to heat shock during this time period; the gene is expressed at the same relatively low level both before and after heat shock. However, in spite of the low level of heat shock mRNA available, globular embryos synthesize the full complement of heat shock proteins in response to heat treatment. The globular embryos appear to accomplish this by translating the existing heat shock mRNAs at an elevated rate, which compensates for the low level of available mRNA. Once the embryos have progressed beyond the globular stage of development, regulation at the transcriptional level resumes, and the embryos again exhibit normal development after heat shock
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.1.12.1137