Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato

We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac wa...

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Bibliographic Details
Published in:The Plant cell 1990-08, Vol.2 (8), p.723-730
Main Author: Yoder, John I.
Format: Article
Language:English
Subjects:
Corn
DNA
DNA probes
DNA Transposable Elements
Gels
Gene Amplification
Gene Rearrangement
Genetic transposition
Genomes
Genomics
Plants
Plants, Genetically Modified
Restriction Mapping
Segregation
Species Specificity
Transposons
Zea mays - genetics
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Summary:We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac was introduced into a transformant, the number of copies usually remained low in subsequent generations. In one lineage, however, the number of Ac elements increased from one to more than 15 copies in only two generations. DNA gel blot analyses indicated that the amplified elements were not grossly rearranged. Amplified copies of Ac resided at unique sites in the genome, and segregation analysis indicated that these sites were not tightly linked at one genetic locus. Taken together, these observations indicate that the mechanism of Ac amplification is associated with transposition.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.2.8.723