Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning

We isolated a complementary DNA sequence for the enzyme sucrose phosphate synthase (SPS) from maize utilizing a limited amino acid sequence. The 3509-bp cDNA encodes a 1068-amino acid polypeptide. The identity of the cDNA was confirmed by the ability of the cloned sequence to direct sucrose phosphat...

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Bibliographic Details
Published in:The Plant cell 1991-10, Vol.3 (10), p.1121-1130
Main Authors: Worrell, A.C. (Calgene Inc., Davis, CA), Bruneau, J.M, Summerfelt, K, Boersig, M, Voelker, T.A
Format: Article
Language:English
Subjects:
ACILTRANSFERASA
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ACYLTRANSFERASE
ALMIDON
AMIDON
Amino Acid Sequence
Amino acids
Base Sequence
Carbohydrate Metabolism
Cloning, Molecular
Complementary DNA
Corn
DNA - genetics
Enzymes
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
GENE
Gene Expression
GENES
Glucosyltransferases - genetics
HOJAS
Leaves
LYCOPERSICON ESCULENTUM
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
Molecular Sequence Data
NUCLEOTIDE
NUCLEOTIDOS
Phosphates
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
Plants - enzymology
Plants - genetics
Plants - metabolism
Polymerase chain reaction
SACCHAROSE
Starches
SUCROSA
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transgenic plants
ZEA MAYS
Zea mays - enzymology
Zea mays - genetics
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Summary:We isolated a complementary DNA sequence for the enzyme sucrose phosphate synthase (SPS) from maize utilizing a limited amino acid sequence. The 3509-bp cDNA encodes a 1068-amino acid polypeptide. The identity of the cDNA was confirmed by the ability of the cloned sequence to direct sucrose phosphate synthesis in Escherichia coli. Because no plant-specific factors were necessary for enzymatic activity, we can conclude that SPS enzyme activity is conferred by a single gene product. Sequence comparisons showed that SPS is distantly related to the enzyme sucrose synthase. When expressed from a ribulose bisphosphate carboxylase small subunit promoter in transgenic tomatoes, total SPS activity was boosted up to sixfold in leaves and appeared to be physiologically uncoupled from the tomato regulation mechanism. The elevated SPS activity caused a reduction of starch and increase of sucrose in the tomato leaves. This result clearly demonstrates that SPS is involved in the regulation of carbon partitioning in the leaves
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.3.10.1121