Arabidopsis SHORT INTEGUMENTS 2 Is a Mitochondrial DAR GTPase

The Arabidopsis short integuments 2-1 (sin2-1) mutant produces ovules with short integuments due to early cessation of cell division in these structures. SIN2 was isolated and encodes a putative GTPase sharing features found in the novel DAR GTPase family. DAR proteins share a signature DAR motif an...

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Bibliographic Details
Published in:Genetics (Austin) 2006-10, Vol.174 (2), p.707-718
Main Authors: Hill, Theresa A, Broadhvest, Jean, Kuzoff, Robert K, Gasser, Charles S
Format: Article
Language:English
Subjects:
Alanine - chemistry
Amino Acid Motifs
Amino Acid Sequence
Apoptosis
Arabidopsis
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - classification
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
Arginine - chemistry
Aspartic Acid - chemistry
Cell Cycle Proteins - genetics
Cell Cycle Proteins - physiology
Flowers & plants
Gene Expression Regulation, Developmental
Genes
GTP Phosphohydrolases - classification
GTP Phosphohydrolases - genetics
GTP Phosphohydrolases - physiology
Humans
Investigations
Kinases
Mitochondria - enzymology
Molecular Sequence Data
Proteins
Ribonuclease III - genetics
Ribonuclease III - physiology
Signal Transduction
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Summary:The Arabidopsis short integuments 2-1 (sin2-1) mutant produces ovules with short integuments due to early cessation of cell division in these structures. SIN2 was isolated and encodes a putative GTPase sharing features found in the novel DAR GTPase family. DAR proteins share a signature DAR motif and a unique arrangement of the four conserved GTPase G motifs. We found that DAR GTPases are present in all examined prokaryotes and eukaryotes and that they have diversified into four paralogous lineages in higher eukaryotes. Eukaryotic members of the SIN2 clade of DAR GTPases have been found to localize to mitochondria and are related to eubacterial proteins that facilitate essential steps in biogenesis of the large ribosomal subunit. We propose a similar role for SIN2 in mitochondria. A sin2 insertional allele has ovule effects similar to sin2-1, but more pronounced pleiotropic effects on vegetative and floral development. The diverse developmental effects of the mitochondrial SIN2 GTPase support a mitochondrial role in the regulation of multiple developmental pathways.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.106.060657