Regulation of pathogenesis-related protein-1a gene expression in tobacco

• Published in: The Plant cell 1993-02, Vol.5 (2), p.159-169
• Main Authors: Uknes, S. (CIBA-GEIGY Corporation, Research Triangle Park, NC), Dincher, S, Friedrich, L, Negrotto, D, Williams, S, Thompson-Taylor, H, Potter, S, Wards, E, Ryals, J
• Format: Article
• Language: English
• Subjects: ACIDE SALICYLIQUE; ACIDO SALICILICO; Adenine - analogs & derivatives; Adenine - pharmacology; ADN RECOMBINADO; ADN RECOMBINE; Agronomy. Soil science and plant productions; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Benzyl Compounds; Biological and medical sciences; CICLOHEXIMIDA; CYCLOHEXIMIDE; Cycloheximide - pharmacology; DNA - genetics; EXPRESION GENICA; EXPRESSION DES GENES; FEUILLE; FLORACION; FLORAISON; Fundamental and applied biological sciences. Psychology; GENE; Gene Deletion; Gene expression; Gene Expression Regulation - drug effects; GENES; GENETICA; GENETIQUE; GLICOSIDASAS; Glucuronidase - genetics; GLYCOSIDASE; HOJAS; Kinetin; Leaves; Lesions; Messenger RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Naphthaleneacetic Acids - pharmacology; Nicotiana - drug effects; Nicotiana - genetics; Nicotiana - microbiology; NICOTIANA TABACUM; PATHOGENESE; PATOGENESIS; Plant cells; Plant Growth Regulators - pharmacology; Plant Proteins - genetics; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; Plants - drug effects; Plants - genetics; Plants - microbiology; Plants, Toxic; POLEN; POLLEN; Promoter Regions, Genetic - drug effects; Protein synthesis; PROTEINAS; PROTEINE; Purines; RACINE; RAICES; Reporter genes; RNA; Salicylates - pharmacology; Salicylic Acid; SINTESIS DE PROTEINAS; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; SYNTHESE PROTEIQUE; TALLO; TIGE; Tobacco Mosaic Virus - pathogenicity; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; VIRUS DE LA MOSAIQUE DU TABAC; VIRUS DEL MOSAICO DEL TABACO
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.5.2.159

Pathogenesis-related protein-1a (Pr-1a) is a protein of unknown function that is strongly induced during the onset of systemic acquired resistance (SAR) in tobacco. The expression of Pr-1a is under complex regulation that is controlled at least partially by the rate of transcription. In this study, we demonstrated that 661 bp of 5' flanking DNA was sufficient to impart tobacco mosaic virus and salicylic acid inducibility to a reporter gene. The Pr-1a promoter did not respond significantly to treatments with either auxin or cytokinin. Experiments with the protein synthesis inhibitor cycloheximide indicated that protein synthesis is required for salicylate-dependent mRNA accumulation. At flowering, the Pr-1a gene was expressed primarily in the mesophyll and epidermal tissues of the leaf blade and the sepals of the flower. Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene