Eukaryotic Lipid Body Proteins in Oleogenous Actinomycetes and Their Targeting to Intracellular Triacylglycerol Inclusions: Impact on Models of Lipid Body Biogenesis

Bacterial neutral lipid inclusions are structurally related to eukaryotic lipid bodies. These lipid inclusions are composed of a matrix of triacylglycerols (TAGs) or wax esters surrounded by a monolayer of phospholipids. Whereas the monolayers of lipid bodies from animal and plant cells harbor speci...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2006-10, Vol.72 (10), p.6743-6750
Main Authors: Hänisch, Jan, Wältermann, Marc, Robenek, Horst, Steinbüchel, Alexander
Format: Article
Language:English
Subjects:
Actinobacteria - metabolism
Adenosine triphosphatase
Animals
Biological and medical sciences
Cellular biology
Eukaryotes
Fundamental and applied biological sciences. Psychology
Inclusion Bodies
Lipid Metabolism
Lipids
Microbiology
Mycobacterium smegmatis
Physiology and Biotechnology
Proteins
Rhodococcus opacus
Studies
Triglycerides - chemistry
Zea mays
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Summary:Bacterial neutral lipid inclusions are structurally related to eukaryotic lipid bodies. These lipid inclusions are composed of a matrix of triacylglycerols (TAGs) or wax esters surrounded by a monolayer of phospholipids. Whereas the monolayers of lipid bodies from animal and plant cells harbor specific classes of proteins which are involved in the structure of the inclusions and lipid homoestasis, no such proteins are known to be associated with bacterial lipid inclusions. The present study was undertaken to reveal whether the mammalian lipid body proteins perilipin A, adipose differentiation-related protein, and tail-interacting protein of 47 kDa (TIP47), which comprise the so called PAT family proteins, and the maize (Zea mays L.) oleosin are targeted to prokaryotic TAG bodies in vivo. When fused to enhanced green fluorescent protein, all proteins except the oleosin were mainly located at the surfaces of lipid inclusions when heterologously expressed in the recombinant actinomycetes Rhodococcus opacus PD630 and Mycobacterium smegmatis mc2155. A more detailed intracellular distribution analysis of TIP47 in recombinant R. opacus cells by immunocytochemical labeling of ultrathin cryosections and freeze fracture replicas revealed a substantial amount of TIP47 protein also pervading the cores of the inclusions. We discuss the impact of these results on the current model of lipid body biogenesis in prokaryotes.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.00584-06