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Expression and function of interleukin‐11 and its receptor α in the human endometrium
The interleukin‐11 (IL‐11) receptor α has an important function in decidualization of mouse endometrial stroma but the function of IL‐11 and its receptor in the human endometrium remains unknown. The mRNA for IL‐11 and its receptor α in human endometrial tissue samples were analysed by semi‐quantita...
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| Published in: | Molecular human reproduction 2003-02, Vol.9 (2), p.75-80 |
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| container_end_page | 80 |
| container_issue | 2 |
| container_start_page | 75 |
| container_title | Molecular human reproduction |
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| creator | Karpovich, Natalia Chobotova, Katya Carver, Janet Heath, John K. Barlow, David H. Mardon, Helen J. |
| description | The interleukin‐11 (IL‐11) receptor α has an important function in decidualization of mouse endometrial stroma but the function of IL‐11 and its receptor in the human endometrium remains unknown. The mRNA for IL‐11 and its receptor α in human endometrial tissue samples were analysed by semi‐quantitative RT–PCR and RNase protection assays respectively. The proteins were detected in frozen endometrial tissue samples by immunofluorescence. The effect of heparin‐binding epidermal growth factor (HB‐EGF) on secretion of IL‐11 by cultured endometrial stromal cells was assessed by enzyme‐linked immunosorbent assay. The proliferative potential of IL‐11 in endometrial stromal cells was assessed by [3H]thymidine uptake. IL‐11 and its receptor α mRNAs and proteins were detected in the endometrium throughout the cycle. Distinct patterns of localization of the ligand and receptor were observed. HB‐EGF induced IL‐11 secretion by cultured stromal cells, and IL‐11 induced [3H]thymidine uptake by these cells. Our data suggest that IL‐11–receptor interactions may perform different functions in the human endometrium at different stages of the cycle, and that secretion of IL‐11 is modulated by local growth factors. |
| doi_str_mv | 10.1093/molehr/gag012 |
| format | article |
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The mRNA for IL‐11 and its receptor α in human endometrial tissue samples were analysed by semi‐quantitative RT–PCR and RNase protection assays respectively. The proteins were detected in frozen endometrial tissue samples by immunofluorescence. The effect of heparin‐binding epidermal growth factor (HB‐EGF) on secretion of IL‐11 by cultured endometrial stromal cells was assessed by enzyme‐linked immunosorbent assay. The proliferative potential of IL‐11 in endometrial stromal cells was assessed by [3H]thymidine uptake. IL‐11 and its receptor α mRNAs and proteins were detected in the endometrium throughout the cycle. Distinct patterns of localization of the ligand and receptor were observed. HB‐EGF induced IL‐11 secretion by cultured stromal cells, and IL‐11 induced [3H]thymidine uptake by these cells. Our data suggest that IL‐11–receptor interactions may perform different functions in the human endometrium at different stages of the cycle, and that secretion of IL‐11 is modulated by local growth factors.</description><identifier>ISSN: 1360-9947</identifier><identifier>ISSN: 1460-2407</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/gag012</identifier><identifier>PMID: 12569176</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Cells, Cultured ; DNA - biosynthesis ; DNA - drug effects ; DNA Primers ; DNA, Complementary - genetics ; Endometrium - cytology ; Endometrium - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Interleukin-11 - genetics ; Interleukin-11 - physiology ; Interleukin-11 Receptor alpha Subunit ; Key words: endometrium/heparin‐binding epidermal growth factor/interleukin‐11/interleukin‐11 receptor α/proliferation ; Mammalian female genital system ; Menstrual Cycle - genetics ; Morphology. Physiology ; Receptors, Interleukin - genetics ; Receptors, Interleukin - physiology ; Receptors, Interleukin-11 ; RNA, Messenger - genetics ; Stromal Cells - cytology ; Stromal Cells - physiology ; Thymidine - metabolism ; Transcription, Genetic ; Vertebrates: reproduction</subject><ispartof>Molecular human reproduction, 2003-02, Vol.9 (2), p.75-80</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-a30d373187566feafd742a3a3814532f701d274019415ed92010f030c384548b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14551974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12569176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karpovich, Natalia</creatorcontrib><creatorcontrib>Chobotova, Katya</creatorcontrib><creatorcontrib>Carver, Janet</creatorcontrib><creatorcontrib>Heath, John K.</creatorcontrib><creatorcontrib>Barlow, David H.</creatorcontrib><creatorcontrib>Mardon, Helen J.</creatorcontrib><title>Expression and function of interleukin‐11 and its receptor α in the human endometrium</title><title>Molecular human reproduction</title><addtitle>Mol. Hum. Reprod</addtitle><description>The interleukin‐11 (IL‐11) receptor α has an important function in decidualization of mouse endometrial stroma but the function of IL‐11 and its receptor in the human endometrium remains unknown. The mRNA for IL‐11 and its receptor α in human endometrial tissue samples were analysed by semi‐quantitative RT–PCR and RNase protection assays respectively. The proteins were detected in frozen endometrial tissue samples by immunofluorescence. The effect of heparin‐binding epidermal growth factor (HB‐EGF) on secretion of IL‐11 by cultured endometrial stromal cells was assessed by enzyme‐linked immunosorbent assay. The proliferative potential of IL‐11 in endometrial stromal cells was assessed by [3H]thymidine uptake. IL‐11 and its receptor α mRNAs and proteins were detected in the endometrium throughout the cycle. Distinct patterns of localization of the ligand and receptor were observed. HB‐EGF induced IL‐11 secretion by cultured stromal cells, and IL‐11 induced [3H]thymidine uptake by these cells. Our data suggest that IL‐11–receptor interactions may perform different functions in the human endometrium at different stages of the cycle, and that secretion of IL‐11 is modulated by local growth factors.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>DNA - biosynthesis</subject><subject>DNA - drug effects</subject><subject>DNA Primers</subject><subject>DNA, Complementary - genetics</subject><subject>Endometrium - cytology</subject><subject>Endometrium - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Interleukin-11 - genetics</subject><subject>Interleukin-11 - physiology</subject><subject>Interleukin-11 Receptor alpha Subunit</subject><subject>Key words: endometrium/heparin‐binding epidermal growth factor/interleukin‐11/interleukin‐11 receptor α/proliferation</subject><subject>Mammalian female genital system</subject><subject>Menstrual Cycle - genetics</subject><subject>Morphology. Physiology</subject><subject>Receptors, Interleukin - genetics</subject><subject>Receptors, Interleukin - physiology</subject><subject>Receptors, Interleukin-11</subject><subject>RNA, Messenger - genetics</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - physiology</subject><subject>Thymidine - metabolism</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: reproduction</subject><issn>1360-9947</issn><issn>1460-2407</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpVkM9K9DAUxYMo6qcu3Uo3Lqu5-dtuBBH_gaILFXETYpvMRNt0SFLRnY_gq3wv4kP4JFZncHR1z-X87rlwENoEvAO4pLtt15hx2B3pEQaygFaBCZwThuXioOmgy5LJFfQvxgeMQRJeLKMVIFyUIMUquj18ngQTo-t8pn2d2d5X6WvpbOZ8MqEx_aPzH69vAN-ASzELpjKT1IXs_f8AZWlssnHfap8ZX3etScH17TpasrqJZmM219D10eHVwUl-dnF8erB_lleMQ8o1xTWVFArJhbBG21oyoqmmBTBOiZUYaiIZhpIBN3VJMGCLKa5owTgr7uka2pvmTvr71tSV8SnoRk2Ca3V4UZ126q_j3ViNuicFgghekCEgnwZUoYsxGPtzC1h9VaymFatpxQO_9fvhnJ51OgDbM0DHSjc2aF-5OOcY51BKNn_sYjLPP74Oj0pIKrk6ub1TN5fiihzdnCtCPwFwxZfo</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Karpovich, Natalia</creator><creator>Chobotova, Katya</creator><creator>Carver, Janet</creator><creator>Heath, John K.</creator><creator>Barlow, David H.</creator><creator>Mardon, Helen J.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20030201</creationdate><title>Expression and function of interleukin‐11 and its receptor α in the human endometrium</title><author>Karpovich, Natalia ; Chobotova, Katya ; Carver, Janet ; Heath, John K. ; Barlow, David H. ; Mardon, Helen J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-a30d373187566feafd742a3a3814532f701d274019415ed92010f030c384548b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>DNA - biosynthesis</topic><topic>DNA - drug effects</topic><topic>DNA Primers</topic><topic>DNA, Complementary - genetics</topic><topic>Endometrium - cytology</topic><topic>Endometrium - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Interleukin-11 - genetics</topic><topic>Interleukin-11 - physiology</topic><topic>Interleukin-11 Receptor alpha Subunit</topic><topic>Key words: endometrium/heparin‐binding epidermal growth factor/interleukin‐11/interleukin‐11 receptor α/proliferation</topic><topic>Mammalian female genital system</topic><topic>Menstrual Cycle - genetics</topic><topic>Morphology. Physiology</topic><topic>Receptors, Interleukin - genetics</topic><topic>Receptors, Interleukin - physiology</topic><topic>Receptors, Interleukin-11</topic><topic>RNA, Messenger - genetics</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - physiology</topic><topic>Thymidine - metabolism</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karpovich, Natalia</creatorcontrib><creatorcontrib>Chobotova, Katya</creatorcontrib><creatorcontrib>Carver, Janet</creatorcontrib><creatorcontrib>Heath, John K.</creatorcontrib><creatorcontrib>Barlow, David H.</creatorcontrib><creatorcontrib>Mardon, Helen J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karpovich, Natalia</au><au>Chobotova, Katya</au><au>Carver, Janet</au><au>Heath, John K.</au><au>Barlow, David H.</au><au>Mardon, Helen J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and function of interleukin‐11 and its receptor α in the human endometrium</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol. Hum. Reprod</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>9</volume><issue>2</issue><spage>75</spage><epage>80</epage><pages>75-80</pages><issn>1360-9947</issn><issn>1460-2407</issn><eissn>1460-2407</eissn><abstract>The interleukin‐11 (IL‐11) receptor α has an important function in decidualization of mouse endometrial stroma but the function of IL‐11 and its receptor in the human endometrium remains unknown. The mRNA for IL‐11 and its receptor α in human endometrial tissue samples were analysed by semi‐quantitative RT–PCR and RNase protection assays respectively. The proteins were detected in frozen endometrial tissue samples by immunofluorescence. The effect of heparin‐binding epidermal growth factor (HB‐EGF) on secretion of IL‐11 by cultured endometrial stromal cells was assessed by enzyme‐linked immunosorbent assay. The proliferative potential of IL‐11 in endometrial stromal cells was assessed by [3H]thymidine uptake. IL‐11 and its receptor α mRNAs and proteins were detected in the endometrium throughout the cycle. Distinct patterns of localization of the ligand and receptor were observed. HB‐EGF induced IL‐11 secretion by cultured stromal cells, and IL‐11 induced [3H]thymidine uptake by these cells. Our data suggest that IL‐11–receptor interactions may perform different functions in the human endometrium at different stages of the cycle, and that secretion of IL‐11 is modulated by local growth factors.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12569176</pmid><doi>10.1093/molehr/gag012</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular human reproduction, 2003-02, Vol.9 (2), p.75-80 |
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| language | eng |
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| source | Oxford Journals Online |
| subjects | Biological and medical sciences Cells, Cultured DNA - biosynthesis DNA - drug effects DNA Primers DNA, Complementary - genetics Endometrium - cytology Endometrium - physiology Female Fundamental and applied biological sciences. Psychology Humans Interleukin-11 - genetics Interleukin-11 - physiology Interleukin-11 Receptor alpha Subunit Key words: endometrium/heparin‐binding epidermal growth factor/interleukin‐11/interleukin‐11 receptor α/proliferation Mammalian female genital system Menstrual Cycle - genetics Morphology. Physiology Receptors, Interleukin - genetics Receptors, Interleukin - physiology Receptors, Interleukin-11 RNA, Messenger - genetics Stromal Cells - cytology Stromal Cells - physiology Thymidine - metabolism Transcription, Genetic Vertebrates: reproduction |
| title | Expression and function of interleukin‐11 and its receptor α in the human endometrium |
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