qPrimerDepot: a primer database for quantitative real time PCR

Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These...

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Bibliographic Details
Published in:Nucleic acids research 2007-01, Vol.35 (suppl-1), p.D805-D809
Main Authors: Cui, Wenwu, Taub, Dennis D, Gardner, Kevin
Format: Article
Language:English
Subjects:
Animals
Databases, Nucleic Acid
DNA Primers
Gene Expression
Humans
Internet
Mice
Reverse Transcriptase Polymerase Chain Reaction - methods
User-Computer Interface
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Summary:Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkl767