A survey of essential gene function in the yeast cell division cycle

Mutations impacting specific stages of cell growth and division have provided a foundation for dissecting mechanisms that underlie cell cycle progression. We have undertaken an objective examination of the yeast cell cycle through flow cytometric analysis of DNA content in TetO(7) promoter mutant st...

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Bibliographic Details
Published in:Molecular biology of the cell 2006-11, Vol.17 (11), p.4736-4747
Main Authors: Yu, Lisa, Peña Castillo, Lourdes, Mnaimneh, Sanie, Hughes, Timothy R, Brown, Grant W
Format: Article
Language:English
Subjects:
Alleles
Cell Size
Chromosomal Proteins, Non-Histone - metabolism
DNA Replication - genetics
Flow Cytometry
G1 Phase - genetics
G2 Phase - genetics
Genes, Essential - genetics
Genes, Fungal - genetics
Nuclear Proteins - metabolism
Nucleocytoplasmic Transport Proteins
Phenotype
Promoter Regions, Genetic - genetics
Protein Transport
S Phase - genetics
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - metabolism
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Summary:Mutations impacting specific stages of cell growth and division have provided a foundation for dissecting mechanisms that underlie cell cycle progression. We have undertaken an objective examination of the yeast cell cycle through flow cytometric analysis of DNA content in TetO(7) promoter mutant strains representing 75% of all essential yeast genes. More than 65% of the strains displayed specific alterations in DNA content, suggesting that reduced function of an essential gene in most cases impairs progression through a specific stage of the cell cycle. Because of the large number of essential genes required for protein biosynthesis, G1 accumulation was the most common phenotype observed in our analysis. In contrast, relatively few mutants displayed S-phase delay, and most of these were defective in genes required for DNA replication or nucleotide metabolism. G2 accumulation appeared to arise from a variety of defects. In addition to providing a global view of the diversity of essential cellular processes that influence cell cycle progression, these data also provided predictions regarding the functions of individual genes: we identified four new genes involved in protein trafficking (NUS1, PHS1, PGA2, PGA3), and we found that CSE1 and SMC4 are important for DNA replication.
ISSN:1059-1524
1939-4586
1059-1524
DOI:10.1091/mbc.e06-04-0368