Determination of Bisphenol A and Related Aromatic Compounds Released from Bis-GMA-Based Composites and Sealants by High Performance Liquid Chromatography

• Published in: Environmental health perspectives 2000-01, Vol.108 (1), p.21-27
• Main Authors: Pulgar, Rosa, Olea-Serrano, M. Fatima, Novillo-Fertrell, Arancha, Rivas, Ana, Pazos, Patricia, Pedraza, Vicente, Navajas, Jose-Manuel, Olea, Nicolas
• Format: Article
• Language: English
• Subjects: Benzhydryl Compounds; Bisphenol A-Glycidyl Methacrylate - chemistry; Bisphenols; Business publications audits; Chromatography, High Pressure Liquid; Commercial products; Elution; Environmental Exposure; Gas Chromatography-Mass Spectrometry; Humans; Leaching; Monomers; Phenols - chemistry; Phenols - pharmacokinetics; Pit and Fissure Sealants - chemistry; Pit and Fissure Sealants - pharmacokinetics; Polymerization; Polymers; Resins; Sealants
• ISSN: 0091-6765; 1552-9924
• DOI: 10.1289/ehp.0010821

Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis-GMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytic methods. Concerns about the estrogenicity of bisphenol A (BPA) and other aromatic components leached from commercial products have been expressed. We studied biphenolic components eluted from seven composites and one sealant before and after in vitro polymerization using HPLC and gas chromatography/mass spectrometry and we investigated how pH modifications affect the leaching of these components. We found BPA (maximal amount 1.8 μg/mg dental material), its dimethacrylate derivative (Bis-DMA, 1.15 μg/mg), bisphenol A diglycidylether (6.1 μg/mg), Bis-GMA (2.0 μg/mg), and ethoxylate and propoxylate of bisphenol A in media in which samples of different commercial products were maintained under controlled pH and temperature conditions. Our results confirm the leaching of estrogenic monomers into the environment by Bis-GMA-based composites and sealants in concentrations at which biologic effects have been demonstrated in in vivo experimental models. The main issue with implications for patient care and dentist responsibility is to further determine the clinical relevance of this estrogenic exposure.