development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods

1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sens...

Full description

Saved in:
Bibliographic Details
Published in:British journal of pharmacology 1975-12, Vol.55 (4), p.503-514
Main Authors: Dighe, K.K, Emslie, H.A, Henderson, L.K, Simon, L, Rutherford, F
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
Antibody Formation
Antibody Specificity
Male
Prostaglandins - immunology
Prostaglandins F - immunology
Rabbits
Radioimmunoassay - methods
Serum Albumin, Bovine
Time Factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a 'solid-phase' system. 4 Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1975.tb07425.x