resource of mapped Dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome

We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify select...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2003-06, Vol.132 (2), p.506-516
Main Authors: Muskett, P.R, Clissold, L, Marocco, A, Springer, P.S, Martienssen, R, Dean, C
Format: Article
Language:English
Subjects:
activator/dissociation transposon
Agrobacterium tumefaciens
Agrobacterium tumefaciens - genetics
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis thaliana
Base Sequence
Biological and medical sciences
chromosome mapping
Corn
DNA Primers
DNA probes
DNA, Bacterial - genetics
DNA, Plant - genetics
Fundamental and applied biological sciences. Psychology
gene targeting
Genes
genetic transformation
Genetic transposition
Genetic vectors
genome
Genome, Plant
Genomes
Genomics
grain crops
Herbicides - pharmacology
insertional mutagenesis
Molecular and cellular biology
Molecular genetics
molecular sequence data
Mutagenesis, Insertional
Mutagenesis. Repair
Nicotiana - genetics
nucleotide sequences
phenotypic variation
plant genetic resources
Plants
Polymerase chain reaction
Research Papers on Systems Biology/Genomics/Bioinformatics
transfer DNA
Transformation, Genetic
transgenic plants
Transposons
Zea mays
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Description
Summary:We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.102.016535