Competitive metabolism of naphthalene, methylnaphthalenes, and fluorene by phenanthrene-degrading pseudomonads

Polynuclear aromatic hydrocarbons (PAHs) typically exist as complex mixtures in contaminated soils, yet little is known about the biodegradation of PAHs in mixtures. We have isolated two physiologically diverse bacteria, Pseudomonas stutzeri P-16 and P. saccharophila P-15, from a creosote-contaminat...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1995-01, Vol.61 (1), p.357-362
Main Authors: Stringfellow, W.T. (University of California, Berkeley, CA.), Aitken, M.D
Format: Article
Language:English
Subjects:
Bacteria
Binding, Competitive
BIODEGRADACION
BIODEGRADATION
Biodegradation, Environmental
Biological and medical sciences
Biology
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
CONTROL DE LA CONTAMINACION
DESINFECTANT
DESINFECTANTES
Enzymes
Fluorenes - metabolism
Fundamental and applied biological sciences. Psychology
HIDROCARBUROS
HIDROCARBUROS AROMATICOS
Hydrocarbons
HYDROCARBURE
HYDROCARBURE AROMATIQUE
LUTTE ANTIPOLLUTION
METABOLISME
METABOLISMO
MINERALISATION
MINERALIZACION
Mission oriented research
Naphthalenes - metabolism
Phenanthrenes - metabolism
Physiology and metabolism
Pollution
POLLUTION DU SOL
POLUCION DEL SUELO
PSEUDOMONAS
Pseudomonas - growth & development
Pseudomonas - metabolism
Pseudomonas stutzeri
SOL ANTHROPOGENE
Spectrophotometry
TIPOS ANTROPOGENICOS DE SUELOS
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Description
Summary:Polynuclear aromatic hydrocarbons (PAHs) typically exist as complex mixtures in contaminated soils, yet little is known about the biodegradation of PAHs in mixtures. We have isolated two physiologically diverse bacteria, Pseudomonas stutzeri P-16 and P. saccharophila P-15, from a creosote-contaminated soil by enrichment on phenanthrene as the sole carbon source and studied their ability to metabolize several other two- and three-ring PAHs. Naphthalene, 1-methylnaphthalene, and 2-methylnaphthalene served as growth substrates for both organisms, while fluorene was only cometabolized. We also studied the effects of these compounds on initial rates of phenanthrene uptake in binary mixtures. Lineweaver-Burk analysis of kinetic measurements was used to demonstrate competitive inhibition of phenanthrene uptake by all four compounds, suggesting that multiple PAHs are being transformed by a common enzyme pathway in whole cells. Estimates of the inhibition coefficient, Ki, are reported for each compound. The occurrence of competitive metabolic processes in physiologically diverse organisms suggests that competitive metabolism may be a common phenomenon among PAH-degrading organisms
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.61.1.357-362.1995