Analysis of four microsatellite markers on the long arm of chromosome 9 by meiotic recombination in flow-sorted single sperm

Meiotic recombination in flow-sorted single sperm was used to analyze four highly polymorphic microsatellite markers on the long arm of chromosome 9. The microsatellites comprised three tightly linked markers: 9CMP1 (D9S109), 9CMP2 (D9S127), and D9S53, which map to 9q31, and a reference marker, ASS,...

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Bibliographic Details
Published in:American journal of human genetics 1993-06, Vol.52 (6), p.1191-1199
Main Authors: FURLONG, R. A, GOUDIE, D. R, CARTER, N. P, LYALL, J. E. W, AFFARA, N. A, FERGUSON-SMITH, M. A
Format: Article
Language:English
Subjects:
Base Sequence
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
BIOLOGICAL INDICATORS
CELL DIVISION
chromosome 9
Chromosome Mapping
CHROMOSOMES
Chromosomes, Human, Pair 9
Classical genetics, quantitative genetics, hybrids
Data Interpretation, Statistical
DNA, Satellite - analysis
Flow Cytometry
Fundamental and applied biological sciences. Psychology
GAMETES
GENE AMPLIFICATION
GENE RECOMBINATION
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
GERM CELLS 550400 > Genetics
Haplotypes
Human
HUMAN CHROMOSOMES
Humans
linkage analysis
Male
man
MAPPING
markers
MEIOSIS
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction
recombination
Recombination, Genetic
RFLPS
SPERMATOZOA
Spermatozoa - ultrastructure
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Summary:Meiotic recombination in flow-sorted single sperm was used to analyze four highly polymorphic microsatellite markers on the long arm of chromosome 9. The microsatellites comprised three tightly linked markers: 9CMP1 (D9S109), 9CMP2 (D9S127), and D9S53, which map to 9q31, and a reference marker, ASS, which is located in 9q34.1. Haplotypes of single sperm were assessed by using PCR in a single-step multiplex reaction to amplify each locus. Recombinant haplotypes were identified by their relative infrequency and were analyzed using THREELOC, a maximum-likelihood-analysis program, and an adaptation of CRI-MAP. The most likely order of these markers was cen-D9S109-D9S127-D9S53-ASS-tel with D9S109, D9S127, and D9S53 being separated by a genetic distance of approximately 3%. The order of the latter three markers did not however achieve statistical significance using the THREELOC program.
ISSN:0002-9297
1537-6605