Selective Inhibition of HIV Replication in Primary Macrophages but Not T Lymphocytes by Macrophage-Derived Chemokine

Macrophage-derived chemokine (MDC) has been reported to inhibit different HIV-1 strains in activated peripheral blood mononuclear cells (T cell blasts), although other investigators have not confirmed these findings. Here we demonstrate that MDC inhibits the replication of CCR5-dependent (R5) HIV-1B...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-08, Vol.97 (16), p.9162-9167
Main Authors: Cota, Manuela, Mengozzi, Manuela, Vicenzi, Elisa, Panina-Bordignon, Paola, Sinigaglia, Francesco, Transidico, Pietro, Sozzani, Silvano, Mantovani, Alberto, Poli, Guido
Format: Article
Language:English
Subjects:
AIDS
AIDS/HIV
Antiviral Agents - physiology
Antiviral Agents - secretion
Base Sequence
Biological Sciences
Blasts
Blood
Cells
Cells, Cultured
Chemokines
Chemokines - physiology
Chemokines - secretion
DNA
DNA Primers
HIV
HIV 1
HIV-1 - physiology
Human immunodeficiency virus
Human immunodeficiency virus 1
Humans
Immunology
Infections
macrophage-derived chemokine
Macrophages
Macrophages - metabolism
Macrophages - virology
T lymphocytes
T-Lymphocytes - virology
Virus Replication
Viruses
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Summary:Macrophage-derived chemokine (MDC) has been reported to inhibit different HIV-1 strains in activated peripheral blood mononuclear cells (T cell blasts), although other investigators have not confirmed these findings. Here we demonstrate that MDC inhibits the replication of CCR5-dependent (R5) HIV-1BaLin monocyte-derived macrophages (MDM), but not in T cell blasts, although with variable potency depending on donor variability. Analysis of HIV-1BaLproviral DNA synthesis in MDM indicated that the suppressive effect of MDC did not involve inhibition of early events such as entry or reverse transcription. Finally, an inverse correlation was observed between the levels of endogenous MDC secreted by uninfected MDM of different donors and the efficiency of different HIV strains, including two primary isolates with different coreceptor usage, to replicate in these cells. Thus, MDC represents an example of a chemokine inhibiting HIV replication in macrophages acting at one or more postentry levels in the virus life cycle.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.160359197