A method of processing first-trimester chorionic villous biopsies for cytogenetic analysis

Chorionic villous biopsy is emerging as a technique for obtaining fetal cells for prenatal diagnosis in the first trimester of pregnancy. Chromosome analysis has been performed on small villous biopsies using either direct harvests of uncultured cells or after culturing villous tissue. Here, we desc...

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Bibliographic Details
Published in:American journal of human genetics 1984-11, Vol.36 (6), p.1386-1393
Main Authors: Blakemore, K J, Watson, M S, Samuelson, J, Breg, W R, Mahoney, M J
Format: Article
Language:English
Subjects:
Biopsy, Needle
Cells, Cultured
Chorionic Villi - pathology
Cytogenetics
Female
Humans
Karyotyping
Pregnancy
Pregnancy Trimester, First
Prenatal Diagnosis - methods
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Summary:Chorionic villous biopsy is emerging as a technique for obtaining fetal cells for prenatal diagnosis in the first trimester of pregnancy. Chromosome analysis has been performed on small villous biopsies using either direct harvests of uncultured cells or after culturing villous tissue. Here, we describe a method where both techniques can be used simultaneously; from a single villous biopsy, GTG-banded chromosomes of improved morphology are obtained from direct preparations and from cultured villous cells.
ISSN:0002-9297
1537-6605