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A method of processing first-trimester chorionic villous biopsies for cytogenetic analysis

Chorionic villous biopsy is emerging as a technique for obtaining fetal cells for prenatal diagnosis in the first trimester of pregnancy. Chromosome analysis has been performed on small villous biopsies using either direct harvests of uncultured cells or after culturing villous tissue. Here, we desc...

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Published in:	American journal of human genetics 1984-11, Vol.36 (6), p.1386-1393
Main Authors:	Blakemore, K J, Watson, M S, Samuelson, J, Breg, W R, Mahoney, M J
Format:	Article
Language:	English
Subjects:	Biopsy, Needle Cells, Cultured Chorionic Villi - pathology Cytogenetics Female Humans Karyotyping Pregnancy Pregnancy Trimester, First Prenatal Diagnosis - methods
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container_title	American journal of human genetics
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creator	Blakemore, K J Watson, M S Samuelson, J Breg, W R Mahoney, M J
description	Chorionic villous biopsy is emerging as a technique for obtaining fetal cells for prenatal diagnosis in the first trimester of pregnancy. Chromosome analysis has been performed on small villous biopsies using either direct harvests of uncultured cells or after culturing villous tissue. Here, we describe a method where both techniques can be used simultaneously; from a single villous biopsy, GTG-banded chromosomes of improved morphology are obtained from direct preparations and from cultured villous cells.
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subjects	Biopsy, Needle Cells, Cultured Chorionic Villi - pathology Cytogenetics Female Humans Karyotyping Pregnancy Pregnancy Trimester, First Prenatal Diagnosis - methods
title	A method of processing first-trimester chorionic villous biopsies for cytogenetic analysis
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