The E7-associated cell-surface antigen: a marker for the 11p13 chromosomal deletion associated with Aniridia-Wilms tumor

Unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to Wilms tumor of the kidney. Utilizing somatic cell hybrids containing either the normal or...

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Bibliographic Details
Published in:American journal of human genetics 1985-09, Vol.37 (5), p.883-889
Main Authors: SCOGGIN, C. H, FISHER, J. H, SHOEMAKER, S. A, MORSE, H, LEIGH, T, RICCARDI, V. M
Format: Article
Language:English
Subjects:
Abnormalities, Multiple - genetics
Animals
Antibodies, Monoclonal
Antigens, Surface - genetics
Biological and medical sciences
Child
Chromosome Banding
Chromosome Deletion
Chromosomes, Human, 6-12 and X
Complex syndromes
Cricetinae
DNA Restriction Enzymes
DNA, Neoplasm - genetics
Fibroblasts - ultrastructure
Genetic Markers
Humans
Hybrid Cells
Iris - abnormalities
Karyotyping
Kidney Neoplasms - genetics
Kidney Neoplasms - immunology
Medical genetics
Medical sciences
Nucleic Acid Hybridization
Wilms Tumor - genetics
Wilms Tumor - immunology
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Summary:Unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to Wilms tumor of the kidney. Utilizing somatic cell hybrids containing either the normal or abnormal chromosome 11 from a child with Wilms tumor and aniridia, we previously mapped the E7 cell-surface antigen to the 11p1300-to-11p15.1 region. To localize even further the site of this antigen on chromosome arm 11p, we have produced somatic cell hybrids from the fibroblasts of a second child with Wilms tumor and aniridia and a different deletion of 11p [46,XY, del (11)(pter---p14.1::p11.2---qter)]. Furthermore, the normal and deleted chromosome 11 could also be distinguished on the basis of a restriction fragment length polymorphism for the beta-globin gene. Hybrid cells containing the deleted chromosome were not killed in the presence of complement and the E7 monoclonal antibody (which recognizes E7 cell surface antigen), while hybrid cells containing the patient's normal chromosome 11 were killed. Thus, expression of the E7-associated cell-surface antigen can be mapped to the 11p13 region, and it appears to be a potential marker of the chromosome abnormality associated with aniridia-Wilms tumor.
ISSN:0002-9297
1537-6605