Monitoring of Stool Microbiota in Subjects with Diarrhea Indicates Distortions in Composition

We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-12, Vol.44 (12), p.4550-4552
Main Authors: Mai, Volker, Braden, Christopher R, Heckendorf, Jill, Pironis, Baiba, Hirshon, Jon Mark
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteriology
Biodiversity
Biological and medical sciences
Diarrhea - microbiology
DNA Fingerprinting - methods
DNA, Bacterial - analysis
DNA, Bacterial - genetics
DNA, Ribosomal - analysis
DNA, Ribosomal - genetics
Electrophoresis, Polyacrylamide Gel - methods
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Genes, rRNA
Humans
Infectious diseases
Intestines - microbiology
Medical sciences
Microbiology
Nucleic Acid Denaturation
Polymerase Chain Reaction
RNA, Ribosomal, 16S - genetics
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Summary:We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity and strong bands indicating the overgrowth of selected bacteria or bacterial groups.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.01542-06