Alternative pathways for DNA-thymine synthesis in stimulated leucocytes

In vitro transformation of stimulated lymphocytes is frequently evaluated from their ability to incorporate thymidine. This technique measures only one pathway by which cells synthesize DNA-thymine. The purpose of this study was to evaluate the incorporation of various precursors into the DNA-thymin...

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Bibliographic Details
Published in:Clinical and experimental immunology 1971-02, Vol.8 (2), p.177-186
Main Author: Wolberg, W H
Format: Article
Language:English
Subjects:
Carbon Isotopes
DNA - biosynthesis
Formates - metabolism
Humans
In Vitro Techniques
Lectins
Leukocytes - immunology
Leukocytes - metabolism
Lymphocyte Activation
Serine - metabolism
Thymidine - metabolism
Thymine - biosynthesis
Tritium
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Summary:In vitro transformation of stimulated lymphocytes is frequently evaluated from their ability to incorporate thymidine. This technique measures only one pathway by which cells synthesize DNA-thymine. The purpose of this study was to evaluate the incorporation of various precursors into the DNA-thymine of stimulated leucocytes. It was found that the uptake of thymidine did provide an adequate assessment of total precursor incorporation.
ISSN:0009-9104
1365-2249