Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin

The cytotoxic titre −1 of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules...

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Bibliographic Details
Published in:Clinical and experimental immunology 1971-12, Vol.9 (6), p.839-851
Main Authors: Woodruff, M F, Inchley, M P
Format: Article
Language:English
Subjects:
Animals
Antibodies, Anti-Idiotypic
Antilymphocyte Serum
Chromatography, DEAE-Cellulose
Complement System Proteins
Cytotoxicity Tests, Immunologic
Goats
Guinea Pigs
Immunization
Immunoelectrophoresis
Immunoglobulin G
Iodine Isotopes
Mice
Rabbits
Thymus Gland - cytology
Thymus Gland - immunology
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Summary:The cytotoxic titre −1 of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with 131 I-ALG, was about 0·002%. When γ 2 IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ 2 preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ 2 . The mechanism of augmentation by γ 2 has been investigated using 131 I-ALG and 125 I- γ 2 IgG.
ISSN:0009-9104
1365-2249